Overview

  • Product name
    Anti-MERTK (phospho Y749 + Y753 + Y754) antibody
    See all MERTK primary antibodies
  • Description
    Rabbit polyclonal to MERTK (phospho Y749 + Y753 + Y754)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CKKIYp SGDYpYpRQG, corresponding to amino acids 746-757 of Human MERTK.

Properties

Applications

Our Abpromise guarantee covers the use of ab14921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/750. Detects a band of approximately 180 kDa.

Target

  • Function
    In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity
    Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
  • Involvement in disease
    Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • c MER antibody
    • c mer proto oncogene tyrosine kinase antibody
    • c-mer antibody
    • cMER antibody
    • cmer protooncogene tyrosine kinase antibody
    • Eyk antibody
    • MER antibody
    • MER receptor tyrosine kinase antibody
    • MERK antibody
    • MERPEN antibody
    • Mertk antibody
    • MERTK c-mer proto-oncogene tyrosine kinase antibody
    • MERTK_HUMAN antibody
    • MGC133349 antibody
    • nmf12 antibody
    • Nyk antibody
    • Proto oncogene tyrosine protein kinase MER antibody
    • Proto oncogene tyrosine protein kinase MER precursor antibody
    • Proto-oncogene c-Mer antibody
    • Receptor tyrosine kinase MerTK antibody
    • RP38 antibody
    • STK kinase antibody
    • Tyrosine-protein kinase Mer antibody
    see all

References

This product has been referenced in:
  • Kariolis MS  et al. Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies. J Clin Invest 127:183-198 (2017). WB ; Human . Read more (PubMed: 27893463) »
  • Jung Y  et al. Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow. Oncotarget 7:25698-711 (2016). WB . Read more (PubMed: 27028863) »
See all 5 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for contacting us.

The actual order number for ab14921 was 1197605. I have sent a new ab52968 with order number 1219455.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question
Answer

Unfortunately this order does not match with anything? Please check again?

Could you provide the address and date when this antibody was purchased?

Thanks!

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Answer

Could you contact the purchasing department for purchase order number?

Thanks!

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Answer

I have requested a retest of this antibody. I will let you know the results when available.

Could you provide the purchase order number of ab14921? The PO will help us to send the ab at the same address we sent before.

Please note without the purchase order number we will not be able to process the order.

Looking forward to hearing from you soon.

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Answer

Thank you for your email.

I am sorry to hear that the results weren't improved. I am happy to replace the antibody with a new one however as this is the only phospho specific antibody we have so there in no option for replacement unless you want a different ab.

In order to know more about the quality of this antibody I have sent your experimental details to my colleague in laboratory. So would you like to wait for the results or you would like to have refund.

Once agian I am sorry for the inconvenience caused. I will look forward to hearing from you soon.

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Answer

Thanks for the information.

The results are very strange. I am sorry I could not find a good reason why the antibodies are showing difference. Technically, they should show the same band size. The MERTK protein is heavily glycosylated so 180 kDa band is possible. Could you tell me which cell line you are using?

Could you redo the experiment without stripping the membrane and by including the no primary control?

Many thanks for your cooperation in this case. I will look forward to hearing from you soon.

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Question

Lot number: 1292976
Inquiry: Hi, I have had a problem with your antibody against phosphorylated MerTK in western blot, it seems to recognise the wrong protein. I have run (Gas-6/Protein S stimulated) cell lysates corresponding to 20 ug total protein (lysis buffer 25 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 10% glycerol, 1% Triton X-100 with protease and phosphatase inhibitors) under reducing conditions on a 4-15% gradient gel followed by blotting to a PVDF membrane. As blocking reagent I used 3% fish gelatine in 50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20, 1 h RT. I incubated the blot with the above mentioned antibody for 1 h at RT diluted 1:1000 in blocking buffer followed by 4 washes with 50 mM Tris pH 8, 150 mM NaCl, 0.1% Tween-20. As a secondary antibody I used a swine anti-rabbit HRP conjugate from Dako, diluted 1:3000 in blocking buffer. Incubation time again 1 h RT. The membrane was washed 4 times as above and developed using ECL. After phosphoMer detection the blot was stripped using 0.4 M NaOH (12 min RT) after which total Mer was analysed using the RabMAb a b52968. When detecting phosphoMer I see a band of approx 130 kDa as opposed to a band of ca 180 kDa when detecting total Mer. This obviously can not be the same protein. Since the antibody against the phosphorylated Mer gives only one band I can assume it is specific, but against another protein. For details, see attached file. Since this antibody is of no use in Mer-studies I was wondering If I can get another antibody as a compensation?

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and would like to ask if you have repeated the experiment by changing any step in protocol. Did you include no primary control?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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