Mesenchymal Stromal Cell Marker (CD44, CD90, Sca-1, CD45, CD29) Antibody Panel - Mouse (ab93759)

Overview

  • Product name

    Mesenchymal Stromal Cell Marker (CD44, CD90, Sca-1, CD45, CD29) Antibody Panel - Mouse
  • Product overview

    Mesenchymal Stromal Cell Marker (CD44, CD90, Sca-1, CD45, CD29) Antibody Panel ab93759 contains mouse reactive antibodies for key mesenchymal stromal cell markers, including a CD44 rat monoclonal, CD90 rat monoclonal, Sca-1 rat monoclonal, CD45 rat monoclonal and CD29 rat monoclonal antibody.


    The Mouse Multipotent Mesenchymal Stromal Cell Marker Antibody Panel is designed for the characterization of cultured or isolated mouse multipotent mesenchymal stromal cells. The panel contains a group of antibodies for the positive (CD44, CD29, Sca-1, CD90) and negative (CD45) selection of this lineage. Multipotent mesenchymal stromal cells (MSCs) represent a heterogeneous subset of stromal cells. They can be bone marrow-derived or isolated from many other different adult tissues including periosteum, trabecular bone, adipose tissue, and synovium. They exhibit the potential to give rise to cells of diverse lineages including adipocytes, chondrocytes and osteocytes.

  • Notes

    Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.

Properties

Images

  • IHC image of ab25340 staining CD44 on Mouse Tissue sections (T-cell lymphoma). The cells were 4% PFA fixed (10 min) and then incubated in Perkin-Elmer TNB Blocking Reagent at 0.5% as blocking agent for 30 mins at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25340, 1/250 dilution) overnight at +4°C. The secondary antibody was ab7097.
  • ICC/IF image of ab51317 staining Sca-1 in mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

References

This product has been referenced in:

  • Mohsen ROM  et al. Role of bone marrow-derived stem cells versus insulin on filiform and fungiform papillae of diabetic albino rats (light, fluorescent and scanning electron microscopic study). Acta Histochem 121:812-822 (2019). Read more (PubMed: 31358295) »
  • Huang X  et al. Rho/Rho-associated protein kinase signaling pathway-mediated downregulation of runt-related transcription factor 2 expression promotes the differentiation of dental pulp stem cells into odontoblasts. Exp Ther Med 15:4457-4464 (2018). Read more (PubMed: 29731830) »
See all 8 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you very much for your interest in Abcam.

Most of the antibodies in this panel has been tested for IHC-P, but not ab3105 against CD90/Thy1. If you wish to try this one for IHC-P you can use the dilution for IHC-Fr, 1-10 ug/ml. You can link to the individual datasheet from the "components" section where it lists each antibody and the applications and look at the specific information for each antibody.

I hope this helps! Please let me know if you have any further questions.

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Answer

Thank you for your reply.

I would be happy to issue you a discount code for ab51317 to test in flow cytometry, the discount code is below.

As for shipping, we ship all of our products on an ice pack and try to keep them as cold as possible. You will need to consult with the airline that you are travelling with to check their conditions for transporting these products. Also, if they are going to brought into another country then you will need to contact the necessary customs agents to ask them if there is any permission required.

I have talked to my colleagues in Customer Service and they have told me that it will not be an issue for you place the order, but have it shipped to your colleagues address.

If there is anything else I can help you with, please let me know.

DISCOUNT CODE:*
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab51317 in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for [Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your reply.

To clarify, will you be placing the order for this product? I ask, as we have to issue the discount code to the person placing the actual order. So if your collaborator is placing the order, then we will need to issue the code under their name. Just check to avoid any confusion later on.

Thank you for your patience and I look forward to your reply.

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Answer

Thank you for your reply.

3 of the 4 antibodies in the kit have are guaranteed to work in flow cytometry on mouse tissue. However, the component antibody ab51317 (anti-Sca1) is untested in this application and so we cannot guarantee that it will work for you in this application.

What I could do, is issue you a testing discount code, to say that you will purchase the Stromal Cell Marker panel (ab93759)and will use it in flow cytometry, but specifically submit feedback on howab51317 works in flow cytometry, in the form of an Abreview. Once you have submitted that feedback, you would be able to use the discount code to get any primary antibody from our catalogue free of charge.

If this is something that you are interested in, then please let me know.

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Answer

Thank you for contacting Abcam.

ab93759 is a Mouse Mesenchymal Stromal Cell Marker Panel containing 50µg of CD44 rat monoclonal, 50µg of CD90 rat monoclonal, 50µg Sca-1 rat monoclonal, 50µg of CD45 rat monoclonal and 50µl of CD29 rabbit monoclonal antibodies.

The number of samples of samples you would be able to characterize using this kit, will be dependent on how much you need to use for your individual experiments.

If there is anything else I can help you with, please let me know.

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Answer

Thank you fopr your inquiry.

This panel was compiled because all antibodies can be used in IHC.

I agree that all antibodies apart from ab51317 (Anti-Sca1 / Ly6A/E antibody [E13 161-7])are tested and guaranteed for flow cytometry and that this application would be a good choice for characterization experiments.

I am sorry I will not be able to extent our guaranteed to all the antibodies for flow cytometry.

But I am happy to offer your customer a testing discount for ab51317 in flow cytometry, if they are interested.

I am sorry I could not be of more help on this occasion. Please let me know if your customer is interested inpurchasing and testing ab51317.

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Answer

Thank you for contacting us.


I have checked the antibodies about suitability with flow, and except the ab51317 all are tested for this application.

As always, if ab51317 should have been unsuitable, we would have mentioned this on the datasheet, therefore, the antibody simply hasn't been tested in Flow. Unfortunately, we cannot offer our testing discount on panels.

Regarding the preferred method, I have asked our Stem cell specialist in this matter and will come back to you as soon as possible.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your inquiry.

Our Mesenchymal Stromal Cell Marker Panels (human: ab93758, mouse: ab93759) consist of five un-conjugated primary antibodies that can be used to characterise mesenchymal stromal cells.

The antibodies are not conjugated and therefore require a secondary antibody with a fluorochrome.

You can choose these fluorochromes according to the FACScan system. Please contact the originator of the FACScan to ask which fluorochromes are suitable/possible to use.

I hope this information is helpful and wish you good luck with your research.

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