Product nameMesenchymal Stromal Cell Marker (CD44, CD90, Sca-1, CD45, CD29) Antibody Panel - Mouse
Mesenchymal Stromal Cell Marker (CD44, CD90, Sca-1, CD45, CD29) Antibody Panel ab93759 contains mouse reactive antibodies for key mesenchymal stromal cell markers, including a CD44 rat monoclonal, CD90 rat monoclonal, Sca-1 rat monoclonal, CD45 rat monoclonal and CD29 rat monoclonal antibody.
The Mouse Multipotent Mesenchymal Stromal Cell Marker Antibody Panel is designed for the characterization of cultured or isolated mouse multipotent mesenchymal stromal cells. The panel contains a group of antibodies for the positive (CD44, CD29, Sca-1, CD90) and negative (CD45) selection of this lineage. Multipotent mesenchymal stromal cells (MSCs) represent a heterogeneous subset of stromal cells. They can be bone marrow-derived or isolated from many other different adult tissues including periosteum, trabecular bone, adipose tissue, and synovium. They exhibit the potential to give rise to cells of diverse lineages including adipocytes, chondrocytes and osteocytes.
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Storage instructionsPlease refer to protocols.
Components 1 units ab25340 - Anti-CD44 antibody [KM201] 1 x 50µg ab25386 - Anti-CD45 antibody [I3/2.3] 1 x 50µg ab3105 - Anti-CD90 / Thy1 antibody [IBL-6/23] 1 x 50µg ab95623 - Anti-Integrin beta 1 antibody [KMI6] 1 x 50µg ab51317 - Anti-Sca1 / Ly6A/E antibody [E13 161-7] 1 x 50µg
IHC image of ab25340 staining CD44 on Mouse Tissue sections (T-cell lymphoma). The cells were 4% PFA fixed (10 min) and then incubated in Perkin-Elmer TNB Blocking Reagent at 0.5% as blocking agent for 30 mins at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25340, 1/250 dilution) overnight at +4°C. The secondary antibody was ab7097.
ICC/IF image of ab51317 staining Sca-1 in mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93759 has been referenced in 8 publications.
- Mohsen ROM et al. Role of bone marrow-derived stem cells versus insulin on filiform and fungiform papillae of diabetic albino rats (light, fluorescent and scanning electron microscopic study). Acta Histochem 121:812-822 (2019). PubMed: 31358295
- Huang X et al. Rho/Rho-associated protein kinase signaling pathway-mediated downregulation of runt-related transcription factor 2 expression promotes the differentiation of dental pulp stem cells into odontoblasts. Exp Ther Med 15:4457-4464 (2018). PubMed: 29731830
- Inubushi T et al. Aberrant perichondrial BMP signaling mediates multiple osteochondromagenesis in mice. JCI Insight 2:N/A (2017). PubMed: 28768899
- Chen TT et al. Anchorage of VEGF to the extracellular matrix conveys differential signaling responses to endothelial cells. J Cell Biol 188:595-609 (2010). PubMed: 20176926
- Berggreen E et al. Characterization of the dental lymphatic system and identification of cells immunopositive to specific lymphatic markers. Eur J Oral Sci 117:34-42 (2009). PubMed: 19196316
- Adams AE et al. Mitochondrial uncoupling protein 1 expression in thymocytes. Biochim Biophys Acta 1777:772-6 (2008). PubMed: 18471433
- Hunt ME & Brown DR Role of sialidase in Mycoplasma alligatoris-induced pulmonary fibroblast apoptosis. Vet Microbiol 121:73-82 (2007). PubMed: 17276629
- Khodadoust MM et al. Complex regulation of Ly-6E gene transcription in T cells by IFNs. J Immunol 163:811-9 (1999). PubMed: 10395674