Key features and details
- Rabbit polyclonal to Mesothelin
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mesothelin antibody
See all Mesothelin primary antibodies
DescriptionRabbit polyclonal to Mesothelin
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
- HeLa and A549 cells; Human colon carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20 - 1/100.|
|IHC-P||1/100 - 1/500.|
FunctionMembrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
Sequence similaritiesBelongs to the mesothelin family.
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Cellular localizationSecreted and Cell membrane. Golgi apparatus.
- Information by UniProt
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
Anti-Mesothelin antibody (ab215438) at 1/300 dilution + Hela lysates
Conjugated secondary antibody at 1/10000 dilution
Flow cytometric analysis of A549 cells labeling Mesothelin with ab215438 at 1/100 dilution for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Mesothelin with ab215438 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab215438 has not yet been referenced specifically in any publications.