Recombinant
RabMAb

Recombinant Anti-Mesothelin antibody [EPR17823-52] (ab187063)

Overview

  • Product name

    Anti-Mesothelin antibody [EPR17823-52]
    See all Mesothelin primary antibodies
  • Description

    Rabbit monoclonal [EPR17823-52] to Mesothelin
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes a different epitope of Mesothelin compared to ab213174.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Mesothelin aa 250-600. The exact sequence is proprietary.
    Database link: Q61468

  • Positive control

    • WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; Mouse lung and heart lysates. IP: NIH/3T3 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa).
IP 1/30.

Target

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similarities

    Belongs to the mesothelin family.
  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Images

  • Lane 1 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution
    Lane 2 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/5000 dilution
    Lane 3 : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/20000 dilution

    All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Mesothelin was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187063 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187063 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 µg (Input). 

    Lane 2: ab187063 IP in NIH/3T3 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187063 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : Mouse heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 40,71 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).

References

ab187063 has not yet been referenced specifically in any publications.

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