Recombinant
RabMAb

Recombinant Anti-Mesothelin antibody [EPR17823-52] - BSA and Azide free (ab232218)

Overview

  • Product name

    Anti-Mesothelin antibody [EPR17823-52] - BSA and Azide free
    See all Mesothelin primary antibodies
  • Description

    Rabbit monoclonal [EPR17823-52] to Mesothelin - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody recognizes a different epitope of Mesothelin compared to ab213174.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse Mesothelin aa 250-600. The exact sequence is proprietary.
    Database link: Q61468

  • Positive control

    • WB: NIH/3T3 whole cell lysate; Mouse lung and heart lysates.
  • General notes

    Ab232218 is the carrier-free version of ab187063. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232218 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similarities

    Belongs to the mesothelin family.
  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Images

  • Mesothelin was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187063 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187063 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 µg (Input). 

    Lane 2: ab187063 IP in NIH/3T3 whole cell lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187063 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187063).

  • All lanes : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : Mouse heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa
    Observed band size: 40,71 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187063).

References

ab232218 has not yet been referenced specifically in any publications.

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