Recombinant Anti-Mesothelin antibody [EPR17823-52] - BSA and Azide free (ab232218)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17823-52] to Mesothelin - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Mesothelin antibody [EPR17823-52] - BSA and Azide free
See all Mesothelin primary antibodies -
Description
Rabbit monoclonal [EPR17823-52] to Mesothelin - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognizes a different epitope of Mesothelin compared to ab213174. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 whole cell lysate; Mouse lung and heart lysates.
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General notes
ab232218 is the carrier-free version of ab187063.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17823-52 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/500.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa).
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IP |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
1/500. |
WB
Use at an assay dependent concentration. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 56047 Mouse
- SwissProt: Q61468 Mouse
- Unigene: 17510 Mouse
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Alternative names
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
see all
Images
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Mesothelin was immunoprecipitated from 1 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab187063 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187063 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).
Lane 2: ab187063 IP in NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab187063 in NIH/3T3 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187063).
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All lanes : Anti-Mesothelin antibody [EPR17823-52] (ab187063) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 40,71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187063).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232218 has not yet been referenced specifically in any publications.