Recombinant Anti-Mesothelin antibody [EPR17823-69] (ab213174)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Mesothelin antibody [EPR17823-69]
See all Mesothelin primary antibodies -
Description
Rabbit monoclonal [EPR17823-69] to Mesothelin -
Host species
Rabbit -
Specificity
This antibody recognizes a different epitope of Mesothelin compared to ab187063. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse Mesothelin aa 250-600. The exact sequence is proprietary.
Database link: Q61468 -
Positive control
- WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; mouse lung lysate. IP: NIH/3T3 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17823-69 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab213174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa). | |
| IP | 1/40. |
Target
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Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 56047 Mouse
- SwissProt: Q61468 Mouse
- Unigene: 17510 Mouse
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Alternative names
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
see all
Images
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![Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)](https://www.abcam.com/ps/products/213/ab213174/Images/ab213174-286620-anti-mesothelin-antibody-epr17823-69-western-blot.jpg)
Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)Lane 1 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/20000 dilution
Lane 2 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/100000 dilution
All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600) 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM /TBST.
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![Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)](https://www.abcam.com/ps/products/213/ab213174/Images/ab213174-286615-anti-mesothelin-antibody-epr17823-69-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)Mesothelin was immunoprecipitated from 1 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab213174 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213174 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: ab213174 IP NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213174 in NIH/3T3 whole cell lysate (-)Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)](https://www.abcam.com/ps/products/213/ab213174/Images/ab213174-286619-anti-mesothelin-antibody-epr17823-69-western-blot.jpg)
Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)All lanes : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 40,71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM /TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).
Datasheets and documents
References
ab213174 has not yet been referenced specifically in any publications.
