Recombinant Anti-Mesothelin antibody [EPR17823-69] (ab213174)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17823-69] to Mesothelin
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-Mesothelin antibody [EPR17823-69]
See all Mesothelin primary antibodies -
Description
Rabbit monoclonal [EPR17823-69] to Mesothelin -
Host species
Rabbit -
Specificity
This antibody recognizes a different epitope of Mesothelin compared to ab187063. -
Tested Applications & Species
Application Species IP MouseWB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; mouse lung lysate. IP: NIH/3T3 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17823-69 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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ELISA pair antibody
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Mouse
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IP |
Mouse
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WB |
Mouse
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Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa).
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IP |
1/40.
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Notes |
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WB
1/1000. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa). |
IP
1/40. |
Target
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Function
Membrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. -
Tissue specificity
Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). -
Involvement in disease
Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. -
Sequence similarities
Belongs to the mesothelin family. -
Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. -
Cellular localization
Secreted and Cell membrane. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 56047 Mouse
- SwissProt: Q61468 Mouse
- Unigene: 17510 Mouse
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Alternative names
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
see all
Images
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Lane 1 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/20000 dilution
Lane 2 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/100000 dilution
All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600) 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer: 5% NFDM /TBST.
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Mesothelin was immunoprecipitated from 1 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab213174 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213174 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2: ab213174 IP NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213174 in NIH/3T3 whole cell lysate (-)Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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All lanes : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 40,71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM /TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab213174 has not yet been referenced specifically in any publications.