RecombinantRabMAb

Recombinant Anti-Mesothelin antibody [EPR17823-69] (ab213174)

Submit a review Submit a question
Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
  • Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
  • Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
  • Anti-Mesothelin antibody [EPR17823-69] (ab213174)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17823-69] to Mesothelin
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Mesothelin antibody [EPR17823-69]
    See all Mesothelin primary antibodies

  • Description

    Rabbit monoclonal [EPR17823-69] to Mesothelin

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes a different epitope of Mesothelin compared to ab187063.

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse mesothelin fragment recombinant protein (aa298-600); NIH/3T3 whole cell lysate; mouse lung lysate. IP: NIH/3T3 whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Mouse
IP
Mouse
WB
Mouse
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa).
IP
1/40.
Notes
WB
1/1000. Detects a band of approximately 71, 40 kDa (predicted molecular weight: 69 kDa).
IP
1/40.

Target

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).

  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.

  • Sequence similarities

    Belongs to the mesothelin family.

  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CAK 1 antibody
      • CAK1 antibody
      • CAK1 antigen antibody
      • cleaved form antibody
      • Megakaryocyte potentiating factor antibody
      • Mesothelin antibody
      • Mesothelin isoform 1 precursor antibody
      • MPF antibody
      • Msln antibody
      • MSLN_HUMAN antibody
      • Pre pro megakaryocyte potentiating factor antibody
      • Pre-pro-megakaryocyte-potentiating factor antibody
      • SMR antibody
      • SMRP antibody
      • Soluble MPF mesothelin related protein antibody
      see all

    Images

    • Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
      Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
      Lane 1 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/20000 dilution
      Lane 2 : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/100000 dilution

      All lanes : His-tagged mouse mesothelin fragment recombinant protein (aa298-600) 10 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
      Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)

      Mesothelin was immunoprecipitated from 1 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab213174 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213174 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

      Lane 1: NIH/3T3 whole cell lysate  10 μg (Input).
      Lane 2: ab213174 IP NIH/3T3 whole cell lysate (+).
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab213174 in NIH/3T3 whole cell lysate (-)

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

    • Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
      Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174)
      All lanes : Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 2 : Mouse lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 69 kDa
      Observed band size: 40,71 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM /TBST.

      The expression profile observed is consistent with what has been described in the literature (PMID: 16857795; PMID: 17332303).

    • Anti-Mesothelin antibody [EPR17823-69] (ab213174)
      Anti-Mesothelin antibody [EPR17823-69] (ab213174)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab213174? Please let us know so that we can cite the reference in this datasheet.

    ab213174 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab213174.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com