Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19025-45] to Mesothelin
- Suitable for: Sandwich ELISA
- Reacts with: Human
Product nameAnti-Mesothelin antibody [EPR19025-45]
See all Mesothelin primary antibodies
DescriptionRabbit monoclonal [EPR19025-45] to Mesothelin
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
FunctionMembrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
Sequence similaritiesBelongs to the mesothelin family.
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Cellular localizationSecreted and Cell membrane. Golgi apparatus.
- Information by UniProt
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
Example of human Mesothelin standard curve in 1X Cell Extraction Buffer PTR using recombinant Mesothelin RabMAb antibody (ab222662) and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.
For a matched antibody pair set, see Human Mesothelin Matched Antibody Pair Kit (ab216051).
For a complete ELISA kit, see Human Mesothelin ELISA kit (ab216168).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab222662 has not yet been referenced specifically in any publications.