Overview

  • Product name

    Anti-Mesothelin antibody [EPR2685(2)]
    See all Mesothelin primary antibodies
  • Description

    Rabbit monoclonal [EPR2685(2)] to Mesothelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Mesothelin aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13421

  • Positive control

    • WB: HeLa whole cell lysate (ab150035); Human fetal lung lysate. IHC-P: Human mesothelioma and ovarian carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40, 69 kDa (predicted molecular weight: 69 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Membrane-anchored forms may play a role in cellular adhesion.
      Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    • Tissue specificity

      Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    • Involvement in disease

      Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
    • Sequence similarities

      Belongs to the mesothelin family.
    • Post-translational
      modifications

      Both MPF and the cleaved form of mesothelin are N-glycosylated.
      Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    • Cellular localization

      Secreted and Cell membrane. Golgi apparatus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAK 1 antibody
      • CAK1 antibody
      • CAK1 antigen antibody
      • cleaved form antibody
      • Megakaryocyte potentiating factor antibody
      • Mesothelin antibody
      • Mesothelin isoform 1 precursor antibody
      • MPF antibody
      • Msln antibody
      • MSLN_HUMAN antibody
      • Pre pro megakaryocyte potentiating factor antibody
      • Pre-pro-megakaryocyte-potentiating factor antibody
      • SMR antibody
      • SMRP antibody
      • Soluble MPF mesothelin related protein antibody
      see all

    Images

    • Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution (purified) + HeLa whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 69 kDa
      Observed band size: 69 kDa
      Additional bands at: 40 kDa (possible cleavage fragment)



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab134109 (purified) at 1/40 immunoprecipitating mesothelin in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 40 and 69 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab134109 at a working dilution of 1/800. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded human mesothelioma with purified ab134109 at a working dilution of 1/800. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • All lanes : Anti-Mesothelin antibody [EPR2685(2)] (ab134109) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 69 kDa

    • Immunohistochemical analysis of paraffin embedded Human mesothelioma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.

    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labelling Mesothelin with unpurified ab134109 at 1/1000.

    • Immunohistochemical analysis of paraffin embedded normal Human pancreas tissue using unpurified ab134109 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Pancreatic carcinoma tissue using unpurified ab134109 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Greator omentum tissue using unpurified ab134109 showing +ve staining.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134109 has not yet been referenced specifically in any publications.

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