Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4509] to Mesothelin
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-Mesothelin antibody [EPR4509]
See all Mesothelin primary antibodies
DescriptionRabbit monoclonal [EPR4509] to Mesothelin
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Recombinant fragment corresponding to residues in Human Mesothelin (UniProt ID: Q13421).
- Human fetal lung, Human breast cancer and HeLa lysates; Human serous ovarian carcinoma tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 69 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||1/100 - 1/500.|
FunctionMembrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
Sequence similaritiesBelongs to the mesothelin family.
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Cellular localizationSecreted and Cell membrane. Golgi apparatus.
- Information by UniProt
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
All lanes : Anti-Mesothelin antibody [EPR4509] (ab133489) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human breast cancer lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Immunohistochemical analysis of Mesothelin in paraffin embedded Human serous ovarian carcinoma tissue, using ab133489 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab133489 has been referenced in 2 publications.
- Regzedmaa O et al. Prevalence of DLL3, CTLA-4 and MSTN Expression in Patients with Small Cell Lung Cancer. Onco Targets Ther 12:10043-10055 (2019). PubMed: 31819500
- Akbari S et al. Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study. Can J Kidney Health Dis 4:2054358117699829 (2017). PubMed: 28540060