Overview

  • Product name

    Anti-Mesothelin antibody [EPR4509]
    See all Mesothelin primary antibodies
  • Description

    Rabbit monoclonal [EPR4509] to Mesothelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human Mesothelin (UniProt ID: Q13421).

  • Positive control

    • Human fetal lung, Human breast cancer and HeLa lysates; Human serous ovarian carcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IHC-P 1/100 - 1/250.
IP 1/100 - 1/500.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Membrane-anchored forms may play a role in cellular adhesion.
      Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    • Tissue specificity

      Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    • Involvement in disease

      Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
    • Sequence similarities

      Belongs to the mesothelin family.
    • Post-translational
      modifications

      Both MPF and the cleaved form of mesothelin are N-glycosylated.
      Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    • Cellular localization

      Secreted and Cell membrane. Golgi apparatus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAK 1 antibody
      • CAK1 antibody
      • CAK1 antigen antibody
      • cleaved form antibody
      • Megakaryocyte potentiating factor antibody
      • Mesothelin antibody
      • Mesothelin isoform 1 precursor antibody
      • MPF antibody
      • Msln antibody
      • MSLN_HUMAN antibody
      • Pre pro megakaryocyte potentiating factor antibody
      • Pre-pro-megakaryocyte-potentiating factor antibody
      • SMR antibody
      • SMRP antibody
      • Soluble MPF mesothelin related protein antibody
      see all

    Images

    • All lanes : Anti-Mesothelin antibody [EPR4509] (ab133489) at 1/1000 dilution

      Lane 1 : Human fetal lung lysate
      Lane 2 : Human breast cancer lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 69 kDa

    • Immunohistochemical analysis of Mesothelin in paraffin embedded Human serous ovarian carcinoma tissue, using ab133489 at a dilution of 1/100.

    References

    This product has been referenced in:

    • Akbari S  et al. Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study. Can J Kidney Health Dis 4:2054358117699829 (2017). Read more (PubMed: 28540060) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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