Overview

  • Product name

    Anti-Mesothelin antibody [MSLN/2131]
    See all Mesothelin primary antibodies
  • Description

    Mouse monoclonal [MSLN/2131] to Mesothelin
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Mesothelin aa 273-407.
    Database link: Q13421

  • Positive control

    • IHC-P: Human lung mesothelioma, endometrial carcinoma and tonsil tissues; Mouse lung tissue; Rat stomach tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab236546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.

Target

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similarities

    Belongs to the mesothelin family.
  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Images

  • ab236546 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Formalin-fixed, paraffin-embedded rat stomach tissue stained for Mesothelin using ab236546 at 2 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded mouse lung tissue stained for Mesothelin using ab236546 at 2 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Mesothelin using ab236546 at 2 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for Mesothelin using ab236546 at 2 μg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung mesothelioma tissue stained for Mesothelin using ab236546 at 2 μg/ml in immunohistochemical analysis.

References

ab236546 has not yet been referenced specifically in any publications.

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