Overview

  • Product name

    Anti-Mesothelin antibody [PPI-2e(IHC)]
    See all Mesothelin primary antibodies
  • Description

    Rabbit monoclonal [PPI-2e(IHC)] to Mesothelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Mesothelin aa 550-650. The exact sequence is proprietary.
    (Peptide available as ab134675)

  • Positive control

    • Fetal liver, uterus cancer, fetal pancreas and ovary cancer lysates.
  • General notes

    This antibody detects full-length Mesothelin precursor (69 kDa) and the C terminal cleaved form of Mesothelin.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab92347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.Can be blocked with Mesothelin peptide (ab134675).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Membrane-anchored forms may play a role in cellular adhesion.
      Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    • Tissue specificity

      Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    • Involvement in disease

      Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
    • Sequence similarities

      Belongs to the mesothelin family.
    • Post-translational
      modifications

      Both MPF and the cleaved form of mesothelin are N-glycosylated.
      Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    • Cellular localization

      Secreted and Cell membrane. Golgi apparatus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAK 1 antibody
      • CAK1 antibody
      • CAK1 antigen antibody
      • cleaved form antibody
      • Megakaryocyte potentiating factor antibody
      • Mesothelin antibody
      • Mesothelin isoform 1 precursor antibody
      • MPF antibody
      • Msln antibody
      • MSLN_HUMAN antibody
      • Pre pro megakaryocyte potentiating factor antibody
      • Pre-pro-megakaryocyte-potentiating factor antibody
      • SMR antibody
      • SMRP antibody
      • Soluble MPF mesothelin related protein antibody
      see all

    Images

    • All lanes : Anti-Mesothelin antibody [PPI-2e(IHC)] (ab92347) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : Uterus cancer lysate
      Lane 3 : Fetal pancreas lysate
      Lane 4 : Ovary cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 69 kDa

    References

    ab92347 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    I have good news today.

    We now added the blocking peptide for ab92347 to our catalog and it is available for purchase.

    ab134675

    https://www.abcam.com/index.html?datasheet=134675 (or use the following: https://www.abcam.com/index.html?datasheet=134675).

    It is notin stock at the moment but has a lead time of 1-2 weeks if ordered now.

    I hope this information is helpful and wish a nice weekend.

    Read More

    Answer

    Thank you for your reply.

    I have to confirm that we do not have this peptide in our catalog at the moment. We are trying toadd this toour catalog but Ido not have any estimationwhen this will be available for your customer.

    Your customer might want to get it synthesized from a other company.

    As for the negative control, any peptide with a different sequence can be used. Your customer most probably already has a unrelated peptide in the freezer.

    I am sorry I could not be of more help on this occasion.

    Read More

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