Key features and details
- Rabbit monoclonal [PPI-2e(IHC)] to Mesothelin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mesothelin antibody [PPI-2e(IHC)]
See all Mesothelin primary antibodies
DescriptionRabbit monoclonal [PPI-2e(IHC)] to Mesothelin
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Mesothelin aa 550-650. The exact sequence is proprietary.
(Peptide available as
- Fetal liver, uterus cancer, fetal pancreas and ovary cancer lysates.
This antibody detects full-length Mesothelin precursor (69 kDa) and the C terminal cleaved form of Mesothelin.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Purification notesThis antibody is not purified. It is provided in cell supernatant & storage buffer
Primary antibody notesThis antibody detects full-length Mesothelin precursor (69 kDa) and the C terminal cleaved form of Mesothelin.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab92347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 69 kDa.Can be blocked with Mesothelin peptide (ab134675).|
FunctionMembrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
Sequence similaritiesBelongs to the mesothelin family.
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Cellular localizationSecreted and Cell membrane. Golgi apparatus.
- Information by UniProt
- CAK 1 antibody
- CAK1 antibody
- CAK1 antigen antibody
All lanes : Anti-Mesothelin antibody [PPI-2e(IHC)] (ab92347) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : Uterus cancer lysate
Lane 3 : Fetal pancreas lysate
Lane 4 : Ovary cancer lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 69 kDa
ab92347 has not yet been referenced specifically in any publications.