Recombinant
RabMAb

Recombinant Anti-Mesothelin antibody [SP74] - C-terminal, prediluted (ab228144)

Overview

  • Product name

    Anti-Mesothelin antibody [SP74] - C-terminal, prediluted
    See all Mesothelin primary antibodies
  • Description

    Rabbit monoclonal [SP74] to Mesothelin - C-terminal, prediluted
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human Mesothelin aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q13421

  • Positive control

    • IHC-P: Human mesothelioma and ovarian adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Pre-diluted / ready to use.

Target

  • Function

    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificity

    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in disease

    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similarities

    Belongs to the mesothelin family.
  • Post-translational
    modifications

    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localization

    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for Mesothelin using ab228144 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for Mesothelin using ab228144 in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Mesothelin using ab228144 in immunohistochemical analysis.

References

ab228144 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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