Anti-Mesp2 antibody [1B3F9] (ab166702)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Mesp2 antibody [1B3F9]
See all Mesp2 primary antibodies -
Description
Mouse monoclonal [1B3F9] to Mesp2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to amino acids 37-94 of Human Mesp2 (UniProt Id: Q0VG99) expressed in E.coli.
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Positive control
- Human Mesp2 recombinant protein; Mesp2 (amino acids 37-94)-hIgGFc transfected HEK293 cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1B3F9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab166702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/2000. Predicted molecular weight: 42 kDa. | |
ELISA | 1/10000. |
Target
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Function
Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. -
Involvement in disease
Defects in MESP2 are the cause of spondylocostal dysostosis type 2 (SCDO2) [MIM:608681]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. -
Sequence similarities
Contains 1 bHLH (basic helix-loop-helix) domain. -
Post-translational
modificationsDegraded by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 145873 Human
- Omim: 605195 Human
- SwissProt: Q0VG99 Human
- Unigene: 37311 Human
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Alternative names
- Basic helix loop helix protein MESP 2 antibody
- Basic helix loop helix protein MESP2 antibody
- BHLH protein MesP2 antibody
see all
Images
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Anti-Mesp2 antibody [1B3F9] (ab166702) at 1/500 dilution + Human Mesp2 recombinant protein
Predicted band size: 42 kDa -
All lanes : Anti-Mesp2 antibody [1B3F9] (ab166702) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Mesp2 (amino acids 37-94)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 42 kDa -
ELISA using ab166702 at a 1/10000 dilution.
Red: Control Antigen (100 ng)
Purple: Antigen (10 ng)
Green: Antigen (50 ng)
Blue: Antigen (100 ng)
Protocols
Datasheets and documents
References
ab166702 has not yet been referenced specifically in any publications.