Anti-Mesp2 antibody [1B3F9] (ab166702)
Key features and details
- Mouse monoclonal [1B3F9] to Mesp2
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mesp2 antibody [1B3F9]
See all Mesp2 primary antibodies -
Description
Mouse monoclonal [1B3F9] to Mesp2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to amino acids 37-94 of Human Mesp2 (UniProt Id: Q0VG99) expressed in E.coli.
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Positive control
- Human Mesp2 recombinant protein; Mesp2 (amino acids 37-94)-hIgGFc transfected HEK293 cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1B3F9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab166702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 42 kDa. | |
| ELISA | 1/10000. |
Target
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Function
Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. -
Involvement in disease
Defects in MESP2 are the cause of spondylocostal dysostosis type 2 (SCDO2) [MIM:608681]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. -
Sequence similarities
Contains 1 bHLH (basic helix-loop-helix) domain. -
Post-translational
modificationsDegraded by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 145873 Human
- Omim: 605195 Human
- SwissProt: Q0VG99 Human
- Unigene: 37311 Human
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Alternative names
- Basic helix loop helix protein MESP 2 antibody
- Basic helix loop helix protein MESP2 antibody
- BHLH protein MesP2 antibody
see all
Images
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![Western blot - Anti-Mesp2 antibody [1B3F9] (ab166702)](https://www.abcam.com/ps/datasheet/images/166/ab166702/Mesp2-Primary-antibodies-ab166702-2.jpg)
Western blot - Anti-Mesp2 antibody [1B3F9] (ab166702)Anti-Mesp2 antibody [1B3F9] (ab166702) at 1/500 dilution + Human Mesp2 recombinant protein
Predicted band size: 42 kDa -
![Western blot - Anti-Mesp2 antibody [1B3F9] (ab166702)](https://www.abcam.com/ps/datasheet/images/166/ab166702/Mesp2-Primary-antibodies-ab166702-3.jpg)
Western blot - Anti-Mesp2 antibody [1B3F9] (ab166702)All lanes : Anti-Mesp2 antibody [1B3F9] (ab166702) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Mesp2 (amino acids 37-94)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 42 kDa -
![ELISA - Anti-Mesp2 antibody [1B3F9] (ab166702)](https://www.abcam.com/ps/datasheet/images/166/ab166702/Mesp2-Primary-antibodies-ab166702-1.jpg)
ELISA - Anti-Mesp2 antibody [1B3F9] (ab166702)ELISA using ab166702 at a 1/10000 dilution.
Red: Control Antigen (100 ng)
Purple: Antigen (10 ng)
Green: Antigen (50 ng)
Blue: Antigen (100 ng)
Datasheets and documents
References (0)
ab166702 has not yet been referenced specifically in any publications.
