Anti-MEST antibody (ab230114)
Key features and details
- Rabbit polyclonal to MEST
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MEST antibody
See all MEST primary antibodies -
Description
Rabbit polyclonal to MEST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human MEST aa 100 to the C-terminus.
Database link: Q5EB52 -
Positive control
- WB: Mouse kidney tissue lysate. IHC-P: Human breast cancer and pancreatic tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39, 38, 34 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 39, 38, 34 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Tissue specificity
Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele. -
Sequence similarities
Belongs to the AB hydrolase superfamily. -
Developmental stage
Monoallelic expression of paternally derived allele was observed in all fetal tissues examined, including brain, skeletal muscle, kidney, adrenal, tongue, heart, skin and placenta. In 75-day fetus, expressed in the amnion, brain, heart, lung, stomach, gut, adrenal, kidney, muscle and liver. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 404180 Cow
- Entrez Gene: 4232 Human
- Entrez Gene: 17294 Mouse
- Entrez Gene: 58827 Rat
- Omim: 601029 Human
- SwissProt: Q2HJM9 Cow
- SwissProt: Q5EB52 Human
- SwissProt: Q07646 Mouse
see all -
Alternative names
- DKFZp686L18234 antibody
- EC 3.-.-.- antibody
- Mesoderm specific transcript (mouse) homolog antibody
see all
Images
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Anti-MEST antibody (ab230114) at 1/1000 dilution + Mouse kidney tissue lysate
Secondary
Goat anti-Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 39, 38, 34 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human breast cancer tissue stained for MEST with ab230114 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreatic tissue stained for MEST with ab230114 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab230114 has been referenced in 2 publications.
- Ruan Z et al. Bone marrow stromal cells-derived microRNA-181-containing extracellular vesicles inhibit ovarian cancer cell chemoresistance by downregulating MEST via the Wnt/ß-catenin signaling pathway. Cancer Gene Ther N/A:N/A (2020). PubMed: 32632270
- Ruan Z & Zhao D Long intergenic noncoding RNA LINC00284 knockdown reduces angiogenesis in ovarian cancer cells via up-regulation of MEST through NF-?B1. FASEB J 33:12047-12059 (2019). PubMed: 31574234