Recombinant Anti-MEST antibody [EPR6970(2)] (ab151564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6970(2)] to MEST
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MEST antibody [EPR6970(2)]
See all MEST primary antibodies -
Description
Rabbit monoclonal [EPR6970(2)] to MEST -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human MEST aa 300-400 (C terminal). The exact sequence is proprietary.
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Positive control
- Human placenta and Human fetal kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6970(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
Target
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Tissue specificity
Highly expressed in hydatidiform moles, but barely expressed in dermoid cysts. Biallelic expression is detected in blood lymphocytes. Seems to imprinted in an isoform-specific manner rather than in a tissue-specific manner in lymphocytes. Isoform 1 is expressed only from the paternal allele. Isoform 2 is expressed from both the paternal allele and the maternal allele. -
Sequence similarities
Belongs to the AB hydrolase superfamily. -
Developmental stage
Monoallelic expression of paternally derived allele was observed in all fetal tissues examined, including brain, skeletal muscle, kidney, adrenal, tongue, heart, skin and placenta. In 75-day fetus, expressed in the amnion, brain, heart, lung, stomach, gut, adrenal, kidney, muscle and liver. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 4232 Human
- Entrez Gene: 17294 Mouse
- Entrez Gene: 58827 Rat
- Omim: 601029 Human
- SwissProt: Q5EB52 Human
- SwissProt: Q07646 Mouse
- SwissProt: Q6P5P5 Rat
- Unigene: 270978 Human
see all -
Alternative names
- DKFZp686L18234 antibody
- EC 3.-.-.- antibody
- Mesoderm specific transcript (mouse) homolog antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab151564 has been referenced in 4 publications.
- Anunciado-Koza RP et al. Social and maternal behavior in mesoderm specific transcript (Mest)-deficient mice. PLoS One 17:e0271913 (2022). PubMed: 35867696
- Wang Y et al. MEST promotes lung cancer invasion and metastasis by interacting with VCP to activate NF-κB signaling. J Exp Clin Cancer Res 40:301 (2021). PubMed: 34560900
- Prasad R et al. Hypermethylation of Mest promoter causes aberrant Wnt signaling in patients with Alzheimer's disease. Sci Rep 11:20075 (2021). PubMed: 34625606
- Leow SC et al. The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis. Development 143:950-61 (2016). PubMed: 26893351