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    met-c-met-antibody-ab137654.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases
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Anti-Met (c-Met) antibody (ab137654)

  • Datasheet
  • SDS
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Western blot - Anti-Met (c-Met) antibody - C-terminal (ab137654)
  • Western blot - Anti-Met (c-Met) antibody (ab137654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody (ab137654)

Key features and details

  • Rabbit polyclonal to Met (c-Met)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Met (c-Met) antibody
    See all Met (c-Met) primary antibodies
  • Description

    Rabbit polyclonal to Met (c-Met)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region witin C terminal amino acids 1329-1390 of Human Met (c-Met).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
WB
1/500 - 1/3000. Predicted molecular weight: 158 kDa.
Notes
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
WB
1/500 - 1/3000. Predicted molecular weight: 158 kDa.

Target

  • Function

    Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
  • Involvement in disease

    Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
    Note=Defects in MET may be associated with gastric cancer.
    Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
    Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
    Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • Domain

    The kinase domain is involved in SPSB1 binding.
  • Post-translational
    modifications

    Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P08581 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4233 Human
    • Omim: 164860 Human
    • SwissProt: P08581 Human
    • Unigene: 132966 Human
    • Alternative names

      • AUTS9 antibody
      • c met antibody
      • D249 antibody
      • Hepatocyte growth factor receptor antibody
      • HGF antibody
      • HGF receptor antibody
      • HGF/SF receptor antibody
      • HGFR antibody
      • MET antibody
      • Met proto oncogene antibody
      • Met proto oncogene tyrosine kinase antibody
      • MET proto oncogene, receptor tyrosine kinase antibody
      • Met proto-oncogene (hepatocyte growth factor receptor) antibody
      • Met proto-oncogene antibody
      • Met protooncogene antibody
      • MET_HUMAN antibody
      • Oncogene MET antibody
      • Par4 antibody
      • Proto-oncogene c-Met antibody
      • RCCP2 antibody
      • Scatter factor receptor antibody
      • SF receptor antibody
      • Tyrosine-protein kinase Met antibody
      see all

    Images

    • Western blot - Anti-Met (c-Met) antibody - C-terminal (ab137654)
      Western blot - Anti-Met (c-Met) antibody - C-terminal (ab137654)
      All lanes : Anti-Met (c-Met) antibody (ab137654) at 1/1000 dilution

      Lane 1 : WT HeLa cell extract
      Lane 2 : Met (c-Met) Knockout HeLa cell extracts

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG

      Predicted band size: 158 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-Met (c-Met) antibody (ab137654)
      Western blot - Anti-Met (c-Met) antibody (ab137654)
      Anti-Met (c-Met) antibody (ab137654) at 1/1000 dilution + Hela S3 whole cell lysate at 30 µg

      Predicted band size: 158 kDa



      5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody (ab137654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody (ab137654)
      Immunohistochemical analysis of paraffin embedded Cal27 xenograft, labelling Met (c-Met) with ab137654 at 1/100 dilution.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab137654? Please let us know so that we can cite the reference in this datasheet.

    ab137654 has been referenced in 2 publications.

    • Endo Y  et al. T-DM1-resistant cells gain high invasive activity via EGFR and integrin cooperated pathways. MAbs 10:1003-1017 (2018). PubMed: 30130447
    • Gerdes MJ  et al. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue. Proc Natl Acad Sci U S A 110:11982-7 (2013). ICC/IF ; Human . PubMed: 23818604

    Customer reviews and Q&As

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