Anti-Met (c-Met) antibody (ab74217)
Key features and details
- Rabbit polyclonal to Met (c-Met)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-Met (c-Met) antibody
See all Met (c-Met) primary antibodies -
Description
Rabbit polyclonal to Met (c-Met) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Met (c-Met) aa 1350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P08581 -
Positive control
- IHC-P: Human ovarian carcinoma tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. -
Involvement in disease
Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
Domain
The kinase domain is involved in SPSB1 binding. -
Post-translational
modificationsDephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4233 Human
- Omim: 164860 Human
- SwissProt: P08581 Human
- Unigene: 132966 Human
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Alternative names
- AUTS9 antibody
- c met antibody
- D249 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab74217 has been referenced in 12 publications.
- Fang QL et al. Long non-coding RNA RP11-284P20.2 promotes cell proliferation and invasion in hepatocellular carcinoma by recruiting EIF3b to induce c-met protein synthesis. Biosci Rep 40:N/A (2020). PubMed: 32100822
- Jiang S et al. KIAA1522 Promotes the Progression of Hepatocellular Carcinoma via the Activation of the Wnt/ß-Catenin Signaling Pathway. Onco Targets Ther 13:5657-5668 (2020). PubMed: 32606779
- Lv WL et al. PAX3 silencing suppresses gastric cancer proliferation and angiogenesis via MET/PI3K signaling. Neoplasma 67:304-311 (2020). PubMed: 31847528
- Du M et al. MicroRNA-200a suppresses migration and invasion and enhances the radiosensitivity of NSCLC cells by inhibiting the HGF/c-Met signaling pathway. Oncol Rep 41:1497-1508 (2019). PubMed: 30569179
- Yin L et al. MicroRNA-365a-3p inhibits c-Rel-mediated NF-?B signaling and the progression of pancreatic cancer. Cancer Lett 452:203-212 (2019). PubMed: 30910589