Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

Reviews (12)Q&A (1)References (67)
Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • ELISA - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
  • Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1454Y] to Met (c-Met) - N-terminal
  • Suitable for: ELISA, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Met (c-Met) antibody [EP1454Y] - N-terminal
    See all Met (c-Met) primary antibodies

  • Description

    Rabbit monoclonal [EP1454Y] to Met (c-Met) - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Met (c-Met) aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P08581
    (Peptide available as ab167073)

  • Positive control

    • WB: Wild-type HAP1 cell lysate. HepG2, HEK-293 and HeLa cell lysate. Mouse and rat thymus tissue lysate. Mouse lung tissue lysate. Hela and A459 whole cell lysate. IHC-P: Human bladder carcinoma and clear cell kidney carcinoma tissue. ICC/IF: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab51067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ELISA
Recombinant fragment
ICC/IF
Human
IHC-P
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 156 kDa).Can be blocked with Human Met (c-Met) peptide (ab167073).
IHC-P (10)
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF (1)
1/100 - 1/250.
Notes
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 156 kDa).Can be blocked with Human Met (c-Met) peptide (ab167073).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.

    • Involvement in disease

      Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
      Note=Defects in MET may be associated with gastric cancer.
      Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
      Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
      Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
      Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family.
      Contains 3 IPT/TIG domains.
      Contains 1 protein kinase domain.
      Contains 1 Sema domain.

    • Domain

      The kinase domain is involved in SPSB1 binding.

    • Post-translational
      modifications

      Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AUTS9 antibody
      • c met antibody
      • D249 antibody
      • Hepatocyte growth factor receptor antibody
      • HGF antibody
      • HGF receptor antibody
      • HGF/SF receptor antibody
      • HGFR antibody
      • MET antibody
      • Met proto oncogene antibody
      • Met proto oncogene tyrosine kinase antibody
      • MET proto oncogene, receptor tyrosine kinase antibody
      • Met proto-oncogene (hepatocyte growth factor receptor) antibody
      • Met proto-oncogene antibody
      • Met protooncogene antibody
      • MET_HUMAN antibody
      • Oncogene MET antibody
      • Par4 antibody
      • Proto-oncogene c-Met antibody
      • RCCP2 antibody
      • Scatter factor receptor antibody
      • SF receptor antibody
      • Tyrosine-protein kinase Met antibody
      see all

    Images

    • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      All lanes : Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 156 kDa
      Observed band size: 240 kDa
      why is the actual band size different from the predicted?



      Low expression:A549 (PMID: 32792859).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

      Immunohistochemical staining of paraffin embedded human bladder carcinoma with purified ab51067 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      PBS was used instead of the primary antibody as the negative control (inset).

    • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      All lanes : Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : MET knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 156 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab51067 observed at 156 kDa. Red - loading control, ab8245 observed at 37 kDa.  

       ab51067 was shown to react with Met (c-Met) in wild-type HeLa. Loss of signal was observed when knockout cell line ab265961 (knockout cell lysate ab256991) was used. Wild-type and Met (c-Met) knockout samples were subjected to SDS-PAGE. ab51067 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Met (c-Met) knockout HAP1 cell lysate (40 µg)
      Lane 3: HepG2 cell lysate (40µg) (40 µg)
      Lane 4: HEK-293 cel lysate (40µg) (40 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab51067 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.

      This western blot image is a comparison between ab51067 and a competitor's top cited rabbit polyclonal antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)This image is courtesy of an Abreview submitted by David Ivancic.

      ab51067 staining Met (c-Met) in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with formaldehyde, permeabilized with 0.05% Tween-20 and blocked for 30 minutes at 22°C; antigen retrieval was by heat mediation in antigen retrieval buffer (100X citrate buffer pH 6.0) (ab94674). Samples were incubated with the primary antibody (1/100) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

      Immunohistochemical staining of paraffin embedded human clear cell kidney carcinoma with purified ab51067 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      PBS was used instead of the primary antibody as the negative control (inset).

    • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      All lanes : Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : Rat thymus tissue lysate
      Lane 3 : Mouse lung tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 156 kDa
      Observed band size: 240 kDa why is the actual band size different from the predicted?



      Lanes 1 - 3: Merged signal (red and green). Green - ab51067 observed at 240 kDa. Red - loading control, ab18058, observed at 130 kDa.

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab51067 and ab18058 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    • ELISA - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      ELISA - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

      ELISA analysis of Human Met recombinant protein at 1000 ng/mL with ab51067. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      All lanes : Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067) at 1/2000 dilution (purified)

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

      Predicted band size: 156 kDa
      Observed band size: 190 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

      Immunofluorescence staining of Jurkat (Human T cell leukemia cell line from peripheral blood) cells with purified ab51067 at a working dilution of 1/100, counterstained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel.

      The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100.

      The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab51067 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: Met (c-Met) knockout HAP1 cell lysate (40 µg)
      Lane 3: HepG2 cell lysate (40µg) (40 µg)
      Lane 4: HEK-293 cel lysate (40µg) (40 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab51067 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab51067 was shown to specifically recognize Met (c-Met) in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when Met (c-Met) knockout samples were used. Wild-type and Met (c-Met) knockout samples were subjected to SDS-PAGE. ab51067 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    • Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)
      Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (ab51067)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (67)

    Publishing research using ab51067? Please let us know so that we can cite the reference in this datasheet.

    ab51067 has been referenced in 67 publications.

    • Kobayashi M  et al. Exploring the potential of engineered exosomes as delivery systems for tumor-suppressor microRNA replacement therapy in ovarian cancer. Biochem Biophys Res Commun 527:153-161 (2020). PubMed: 32446360
    • Tang H  et al. Delayed recanalization after MCAO ameliorates ischemic stroke by inhibiting apoptosis via HGF/c-Met/STAT3/Bcl-2 pathway in rats. Exp Neurol 330:113359 (2020). PubMed: 32428505
    • Smith DC  et al. Efficacy and Effect of Cabozantinib on Bone Metastases in Treatment-naive Castration-resistant Prostate Cancer. Clin Genitourin Cancer N/A:N/A (2020). PubMed: 32299729
    • Ueki R  et al. A chemically unmodified agonistic DNA with growth factor functionality for in vivo therapeutic application. Sci Adv 6:eaay2801 (2020). PubMed: 32270033
    • Qiu SQ  et al. High hepatocyte growth factor expression in primary tumor predicts better overall survival in male breast cancer. Breast Cancer Res 22:30 (2020). PubMed: 32188473
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 (ab94674)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Lung
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 (ab94674)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 (ab94674)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Kidney H9 Organoid)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Kidney H9 Organoid
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 (ab94674)
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Liver
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (xenograft with human tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    xenograft with human tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (xenograft with human breast tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    xenograft with human breast tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Breast tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 25 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Met (c-Met) antibody [EP1454Y]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 04 2017

    1-10 of 13 Abreviews or Q&A

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