Overview

  • Product name
    Anti-Met Enkephalin antibody
  • Description
    Rabbit polyclonal to Met Enkephalin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Rmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Met Enkephalin conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde). The conjugate was dialysed to remove excess conjugating agent prior to immunisation.

  • Positive control
    • Typical test tissues included rat and human brain, rat spinal cord, pig duodenum, guinea pig ileum (enteric nervous system) and human phaeochromocytoma (adrenomedullary tumour)

Properties

Applications

Our Abpromise guarantee covers the use of ab22620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-R 1/500 - 1/4000. (Using overnight incubation and ABC/PAP procedure). Dilutions up to 1/10,000 have been achieved under optimised conditions. 1/200-1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended. Frozen sections and Vibratome slices are recommended for tracttracing.

Target

  • Function
    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
  • Sequence similarities
    Belongs to the opioid neuropeptide precursor family.
  • Post-translational
    modifications
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Leu-enkephalin antibody
    • Met-enkephalin antibody
    • Met-enkephalin-Arg-Gly-Leu antibody
    • Met-enkephalin-Arg-Phe antibody
    • Methionine enkephalin antibody
    • OGF antibody
    • Opioid growth factor antibody
    • PENK antibody
    • PENK(114-133) antibody
    • PENK(143-183) antibody
    • PENK(237-258) antibody
    • PENK_HUMAN antibody
    • PPA antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody
    • Proenkephalin-A antibody
    • Synenkephalin antibody
    see all

Images

  • ICC/IF image of ab22620 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22620, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab22620 (4µg/ml) staining met enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Hollis F  et al. Mitochondrial function in the brain links anxiety with social subordination. Proc Natl Acad Sci U S A 112:15486-91 (2015). IHC-Fr ; Rat . Read more (PubMed: 26621716) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Thymic epithelial cells)
Permeabilization
Yes - 1% Triton
Specification
Thymic epithelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Sucrose

Miss. Noam Kadouri

Verified customer

Submitted Oct 11 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain slices (Striatum))
Permeabilization
Yes - 15 minutes in PBS-Triton 0.2%
Specification
Brain slices (Striatum)
Blocking step
PBS-Tween 0.05% + Goat Serum 10% + 0.3M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 23 2018

Answer

Thank you for your inquiry.

I can confirm that ab22620 was tested and is guaranteed for human samples.

If your customer would like to use this antibody with another species, I will be happy to issue a testing discount.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions or if your customer would like to test this antibody in another species.

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Answer

Thanks for your call today. I ran a BLAST with the immunogen of ab98128 against the Met and Leu sequences on SwissProt, and though the immunogen contains the entire Met sequence (YGGFM), it does not contain the full Leu sequence (only YGGF). So, this antibody might not react as strongly with the Leu enkephalin. Since none of our enkephalin antibodies have been tested in perfusion-fixed monkey tissue, I can offer a discount off a future purchase if you buy one of these antibodies now, test it in IHC-FoFr with monkey tissue, and submit feedback to us in the form of an Abreview. The discount is worth 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test one of these antibodies. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering. 2. Purchase the antibody either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-FoFr on monkey tissue. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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