Product nameAnti-Metabotropic Glutamate Receptor 1a antibody
See all Metabotropic Glutamate Receptor 1a primary antibodies
DescriptionRabbit polyclonal to Metabotropic Glutamate Receptor 1a
SpecificityIHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species.
Tested applicationsSuitable for: ICC/IF, WB, IHC (PFA fixed)more details
Species reactivityReacts with: Rat, Human
Predicted to work with: Cynomolgus monkey
- Rat brain (singulate cortex) Human brain lysate Rat cerebellar lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab51314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC (PFA fixed)|
Immunohistochemistry (PFA fixed): 1/250.
WB: 1/1000. Predicted molecular weight: 133 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
Tissue specificityDetected in brain.
Involvement in diseaseSpinocerebellar ataxia, autosomal recessive, 13
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- Glutamate receptor metabotropic 1 antibody
- GPRC1A antibody
- GRM1 alpha antibody
Metabotropic Glutamate Receptor 1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. Metabotropic Glutamate Receptor 1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg
AP-conjugated secondary at 1/5000 dilution
Predicted band size: 133 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
All lanes : Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)
Lane 1 : Rat cerebellar membranes
Lane 2 : Rat cerebellar membranes + DTT
Predicted band size: 133 kDa
Observed band size: 133 kDa
This product has been referenced in:
- Gruol DL et al. Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6. J Neuroimmunol 239:28-36 (2011). WB ; Mouse . Read more (PubMed: 21890220) »
- Romano C et al. Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69 (1995). Read more (PubMed: 7636025) »