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RecombinantRabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

Reviews (1) Submit a question References (5)
Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
  • Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 + Metabotropic Glutamate Receptor 3/MGLUR3
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975]
    See all Metabotropic Glutamate Receptor 2/MGLUR2+Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 + Metabotropic Glutamate Receptor 3/MGLUR3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Mouse primary neurone cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/1800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at dilution between 1/100 to 1/250
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 95 kDa.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/70.
Notes
IHC-P
1/1800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at dilution between 1/100 to 1/250
WB
1/10000 - 1/50000. Predicted molecular weight: 95 kDa.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/70.

Target

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    All lanes : Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution

    Lane 1 : HEK-293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc/flag-tag, not boiled whole cell lysate
    Lane 2 : HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR2 (aa1-872) with myc/flag-tag, not boiled whole cell lysate
    Lane 3 : HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR3 (aa1-879) with myc/flag-tag, not boiled whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 95 kDa
    Observed band size: 250,95 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking buffer: 5% NFDM/TBST.

  • Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution (Purified) + Human brain lysate unboiled

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 95 kDa

  • Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    All lanes : Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution (Purified)

    Lane 1 : Mouse brain lysate boiled
    Lane 2 : Mouse brain lysate unboiled
    Lane 3 : Rat brain lysate boiled
    Lane 4 : Rat brain lysate unboiled

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:1800 (0.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:1800 (0.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:1800 (0.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Flow Cytometry analysis of Mouse primary neuron cells cells labelling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1:70 dilution (10 ?g/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

    Immunocytochemistry analysis of mouse primary neurone cells labelling MGLUR2 with ab150387 at 2 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 rabbit monoclonal antibody (ab11267) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue). 

    Confocal image showing positive staining in mouse primary neuron cells.

    Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

  • Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)
    Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387)

References (5)

Publishing research using ab150387? Please let us know so that we can cite the reference in this datasheet.

ab150387 has been referenced in 5 publications.

  • Dean B  et al. Changes in levels of cortical metabotropic glutamate 2 receptors with gender and suicide but not psychiatric diagnoses. J Affect Disord 244:80-84 (2019). PubMed: 30326345
  • Rogers EJ  et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). PubMed: 30842726
  • Habrian CH  et al. Conformational pathway provides unique sensitivity to a synaptic mGluR. Nat Commun 10:5572 (2019). PubMed: 31804469
  • Wang J  et al. Metabotropic glutamate receptor subtype 2 is a cellular receptor for rabies virus. PLoS Pathog 14:e1007189 (2018). PubMed: 30028877
  • Molosh AI  et al. Panic results in unique molecular and network changes in the amygdala that facilitate fear responses. Mol Psychiatry N/A:N/A (2018). PubMed: 30108314

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Western blot abreview for Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain lysate)
Gel Running Conditions
Reduced Denaturing (sample heated at 37 degrees for 5 minutes, 4%-15% gel)
Loading amount
10 µg
Specification
Brain lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Oct 11 2016

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