Product nameAnti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s]
See all Metabotropic Glutamate Receptor 2 primary antibodies
DescriptionMouse monoclonal [mG2Na-s] to Metabotropic Glutamate Receptor 2
SpecificityThis antibody recognizes the metabotrophic glutamate receptor 2, but not metabotrophic glutamate receptor 3, in rat and mouse.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Fusion protein corresponding to Metabotropic Glutamate Receptor 2. Fusion protein contains a 47 amino acid sequence of the N terminal of the Metabotrophic Glutamate Receptor 2
- WB: Rat brain lysate. IHC: Mouse cortical neurons.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 0.02% Potassium chloride, 0.04% Potassium phosphate, 0.81% Sodium chloride, 0.16% Sodium phosphate
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab15672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 104 kDa). See reference Neki et al, Neurosci Letts.|
FunctionReceptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
Tissue specificityWidely expressed in different regions of the adult brain as well as in fetal brain.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- AMPA selective glutamate receptor 2 antibody
- GLUR2 antibody
- GLURB antibody
Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s] (ab15672) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 99 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 99 kDa could potentially be a cleaved form of Metabotropic Glutamate Receptor 2 due to the presence of an 18 amino acid signal peptide. Metabotropic Glutamate Receptor 2 also contains a number of potential glycosylation sites (SwissProt) which may explain the fuzzy banding pattern observed.
Immunohistochemical analysis of mouse cortical neurons, staining Metabotropic Glutamate Receptor 2 with ab15672. Donkey anti-mouse 6-nm colloidal gold particles were used to detect staining. Images were visualized by electron microscopy.
This product has been referenced in:
- Ji Y et al. Opposing Roles of Estradiol and Testosterone on Stress-Induced Visceral Hypersensitivity in Rats. J Pain 19:764-776 (2018). WB ; Rat . Read more (PubMed: 29496640) »
- Cao DY et al. EXPRESS: Histone hyperacetylation modulates spinal type II metabotropic glutamate receptor alleviating stress-induced visceral hypersensitivity in female rats. Mol Pain 12:N/A (2016). WB . Read more (PubMed: 27385724) »