Product nameAnti-Metabotropic Glutamate Receptor 2+3 antibody
DescriptionRabbit polyclonal to Metabotropic Glutamate Receptor 2+3
This antibody recognises the monomer version (110kDa) and the dimer (220kDa) of Metabotropic Glutamate Receptor 2+3.
Tested applicationsSuitable for: IHC-P, IHC-Fr, WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Cat
Predicted to work with: Mammals
This antibody was raised against a synthetic peptide corresponding to the 13 c-terminus amino acids of mGluR2/3 with an N-appended lysine coupled to thyroglobulin using glutaraldehyde.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab6438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000. Detects a band of approximately 100, 200 kDa.|
RelevanceL glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. The activity of GRM2 and GRM3 receptors are mediated by a G-protein that inhibits adenylate cyclase activity.
Cellular localizationIntegral membrane protein.
- GLUR2 antibody
- GLUR3 antibody
- Glutamate receptor homolog antibody
ab6438 staining Metabotropic Glutamate Receptor 2+3 in rat retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, blocked using 10% serum for 20 minutes at 24°C, then incubated with ab6438 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
GCL - ganglion cell layerIPL - inner plexiform layer
INL - inner nuclear layerOPL - outer plexiform layer
ONL - outer nuclear layer
ab6438 staining the Metabotropic Glutamate Receptor 2+3 in Chicken brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 5% serum for 1 hour 30 minutes at 20°C. The sample was incubated with primary antibody (1/100) at 4°C for 8 hours. Ab6720 (1/400) was used as secondary antibody.
Anti-Metabotropic Glutamate Receptor 2+3 antibody (ab6438) at 1/1000 dilution + rat hippocampal lysate at 10 µg
showing the specific immunolabeling of the ~110k monomer and the ~220k dimer of Metabotropic Glutamate Receptor 2+3
This product has been referenced in:
- Goniotaki D et al. Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity. PLoS Pathog 13:e1006733 (2017). Read more (PubMed: 29176838) »
- Amat SB et al. Using c-kit to genetically target cerebellar molecular layer interneurons in adult mice. PLoS One 12:e0179347 (2017). IHC ; Mouse . Read more (PubMed: 28658323) »