Recombinant
RabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975]
    See all Metabotropic Glutamate Receptor 2/MGLUR2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 2/MGLUR2 aa 850-950 (C terminal). The immunogen used for this product shares 92% homology with Metabotropic glutamate receptor 3. Cross-reactivity with this protein has not been confirmed experimentally.

  • Positive control

    • Human hypothalamus, cerebellum and fetal forebrain cell lysates; Human fetal brain tissue; SH-SY-SY cells
  • General notes

     

     

     This product was previously labelled as Metabotropic Glutamate Receptor 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8975
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 95 kDa.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
    • Tissue specificity

      Widely expressed in different regions of the adult brain as well as in fetal brain.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 3 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMPA selective glutamate receptor 2 antibody
      • GLUR2 antibody
      • GLURB antibody
      • Glutamate metabotropic receptor 2 antibody
      • Glutamate receptor homolog antibody
      • Glutamate receptor metabotropic 2 antibody
      • GPRC1B antibody
      • GRM2 antibody
      • GRM2_HUMAN antibody
      • Metabotropic glutamate receptor 2 antibody
      • mGlu2 antibody
      • mGluR2 antibody
      • OTTHUMP00000210984 antibody
      • OTTHUMP00000210986 antibody
      see all

    Images

    • All lanes : Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387) at 1/10000 dilution

      Lane 1 : Human hypothalamus cell lysate
      Lane 2 : Human fetal brain cell lysate
      Lane 3 : Human cerebellum cell lysate
      Lane 4 : Human fetal forebrain cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 95 kDa

    • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150387 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150387 showing -ve staining.

    • Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Metabotropic Glutamate Receptor 2/MGLUR2 with unpurified ab150387 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of Human fetal brain tissue labelling Metabotropic Glutamate Receptor 2/MGLUR2 with ab150387 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab150387 showing -ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab150387 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Fetal kidney tissue using ab150387 showing -ve staining.

    References

    This product has been referenced in:

    • Dean B  et al. Changes in levels of cortical metabotropic glutamate 2 receptors with gender and suicide but not psychiatric diagnoses. J Affect Disord 244:80-84 (2019). Read more (PubMed: 30326345) »
    • Rogers EJ  et al. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Front Mol Neurosci 12:43 (2019). Read more (PubMed: 30842726) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Brain lysate)
    Gel Running Conditions
    Reduced Denaturing (sample heated at 37 degrees for 5 minutes, 4%-15% gel)
    Loading amount
    10 µg
    Specification
    Brain lysate
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 11 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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