Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637)

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6]
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies
  • Description

    Mouse monoclonal [7A5A6] to Metabotropic Glutamate Receptor 3/MGLUR3
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Metabotropic Glutamate Receptor 3/MGLUR3 aa 433-576 (extracellular).
    Database link: Q14832

  • Positive control

    • WB: Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576)-hIgGFc transfected HEK-293 cell lysate; Human Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576) recombinant protein. ICC/IF: HeLa cells. Flow Cytometry: SH-SY5Y cells. IHC-P: Human cervical cancer tissue.
  • General notes

     This product was previously labelled as Metabotropic Glutamate Receptor 3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab233637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 99 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.
ICC/IF 1/200 - 1/1000.

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GLUR3 antibody
    • Glutamate metabotropic receptor 3 antibody
    • Glutamate receptor metabotropic 3 antibody
    • GPRC1C antibody
    • GRM3 antibody
    • GRM3_HUMAN antibody
    • Metabotropic glutamate receptor 3 antibody
    • MGlu3 antibody
    • mGluR3 antibody
    see all

Images

  • All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 99 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Metabotropic Glutamate Receptor 3/MGLUR3 (green) using ab233637 at 1/200 dilution in ICC/IF. Secondary antibody: Goat anti-Mouse IgG (H+L) DyLight 488. 

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Paraffin-embedded human cervical cancer tissue stained for Metabotropic Glutamate Receptor 3/MGLUR3 using ab233637 at 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Metabotropic Glutamate Receptor 3/MGLUR3 with ab233637 at 1/200 dilution (green) compared with a negative control (red).

  • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) at 1/500 dilution + Human Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576) recombinant protein

    Predicted band size: 99 kDa



    Expected MW is 42.4 kDa.

References

ab233637 has not yet been referenced specifically in any publications.

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