Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies
  • Description

    Rabbit polyclonal to Metabotropic Glutamate Receptor 3/MGLUR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Mouse Metabotropic Glutamate Receptor 3/MGLUR3 aa 400-500 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Human Brain; Mouse Hippocampus; Rat Hippocampus; Mouse Cerebellum; Rat Cerebellum. IHC-P: FFPE Mouse brain tissue sections.
  • General notes

     This product was previously labelled as Metabotropic Glutamate Receptor 3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab140741 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).

Target

Images

  • IHC image of Metabotropic Glutamate Receptor 3/MGLUR3 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140741, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody (ab140741) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Mouse Hippocampus Tissue Lysate
    Lane 5 : Rat Hippocampus Tissue Lysate
    Lane 6 : Mouse Cerebellum Tissue Lysate
    Lane 7 : Rat Cerebellum Neuronal cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 99 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    The band observed at 95 kDa could potentially be a cleaved form of Metabotropic Glutamate Receptor 3/MGLUR3 due to the presence of a 22 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140741 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Xing B  et al. Juvenile treatment with mGluR2/3 agonist prevents schizophrenia-like phenotypes in adult by acting through GSK3ß. Neuropharmacology 137:359-371 (2018). WB . Read more (PubMed: 29793154) »
  • Marques S  et al. Oligodendrocyte heterogeneity in the mouse juvenile and adult central nervous system. Science 352:1326-9 (2016). Read more (PubMed: 27284195) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 09 2016

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