Recombinant Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24635-17] to Metabotropic Glutamate Receptor 3/MGLUR3
Suitable for: WB, IHC-Fr, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17]
See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies
Description

Rabbit monoclonal [EPR24635-17] to Metabotropic Glutamate Receptor 3/MGLUR3
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Fr, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain and Rat brain tissue lysate IHC-Fr: Mouse cerebrum and Rat cerebrum tissue Flow Cyt: Mouse primary neuron and Rat primary neuron, 293T cells transfected with myc tagged human MGLUR3 construct. IP: Mouse brain and Rat brain.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24635-17
Isotype

IgG
Research areas
- Neuroscience
- Processes

Compatible Secondaries
Isotype control
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Predicted molecular weight: 99 kDa.
IHC-Fr		1/100.
Flow Cyt		1/500.
IP		1/30.

Notes
WB 1/1000. Predicted molecular weight: 99 kDa.
IHC-Fr 1/100.
Flow Cyt 1/500.
IP 1/30.

Application notes

Is unsuitable for ICC/IF or IHC-P.

Function

Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
Sequence similarities

Belongs to the G-protein coupled receptor 3 family.
Cellular localization

Cell membrane.
Target information above from: UniProt accession Q14832 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2913 Human
- Entrez Gene: 108069 Mouse
- Entrez Gene: 24416 Rat
- Omim: 601115 Human
- SwissProt: Q14832 Human
- SwissProt: Q9QYS2 Mouse
- SwissProt: P31422 Rat
- Unigene: 590575 Human
- Unigene: 318966 Mouse
- Unigene: 41715 Rat
see all
Alternative names
- GLUR3 antibody
- Glutamate metabotropic receptor 3 antibody
- Glutamate receptor metabotropic 3 antibody
- GPRC1C antibody
- GRM3 antibody
- GRM3_HUMAN antibody
- Metabotropic glutamate receptor 3 antibody
- MGlu3 antibody
- mGluR3 antibody
see all

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 99 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: kidney, testis (PMID: 8840013).

The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time: 136 seconds
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml, Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml, Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/m, Green). The nuclear counterstain was DAPI (Blue). No staining on mouse liver.

Negative control: liver (PMID: 9001707) is observed.

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml, Green). The nuclear counterstain was DAPI (Blue). No staining on rat liver.

Negative control: liver (PMID: 9001707) is observed.

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Flow cytometric analysis of mouse primary neuron cells labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1µg / Right) compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. Goat F(ab')2 Anti-Rabbit IgG(DyLight^® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Flow cytometric analysis of rat primary neuron cells labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1µg / Right) compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. Goat F(ab')2 Anti-Rabbit IgG(DyLight^® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T (Human embryonic kidney epithelial cell) cells transfected with myc tagged human MGLUR3 construct labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1µg / Right), compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. A Goat F(ab')2 Anti-Rabbit IgG(DyLight^® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG or ab283572. Then fixed with 2% PFA for 10 minutes followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor^®647.
Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Metabotropic glutamate receptor 3/MGLUR3 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab283572 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283572 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse brain tissue lysate 10µg

Lane 2: ab283572 IP in Mouse brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283572 in mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds
Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

Metabotropic glutamate receptor 3/MGLUR3 was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab283572 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283572 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Rat brain tissue lysate 10µg

Lane 2: ab283572 IP in Rat brain tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283572 in rat brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds
Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab283572? Please let us know so that we can cite the reference in this datasheet.

ab283572 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As