Recombinant Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24635-17] to Metabotropic Glutamate Receptor 3/MGLUR3
- Suitable for: WB, IHC-Fr, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17]
See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies -
Description
Rabbit monoclonal [EPR24635-17] to Metabotropic Glutamate Receptor 3/MGLUR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and Rat brain tissue lysate IHC-Fr: Mouse cerebrum and Rat cerebrum tissue Flow Cyt: Mouse primary neuron and Rat primary neuron, 293T cells transfected with myc tagged human MGLUR3 construct. IP: Mouse brain and Rat brain.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24635-17 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 99 kDa.
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IHC-Fr |
1/100.
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Flow Cyt |
1/500.
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IP |
1/30.
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Notes |
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WB
1/1000. Predicted molecular weight: 99 kDa. |
IHC-Fr
1/100. |
Flow Cyt
1/500. |
IP
1/30. |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2913 Human
- Entrez Gene: 108069 Mouse
- Entrez Gene: 24416 Rat
- Omim: 601115 Human
- SwissProt: Q14832 Human
- SwissProt: Q9QYS2 Mouse
- SwissProt: P31422 Rat
- Unigene: 590575 Human
see all -
Alternative names
- GLUR3 antibody
- Glutamate metabotropic receptor 3 antibody
- Glutamate receptor metabotropic 3 antibody
see all
Images
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All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 99 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: kidney, testis (PMID: 8840013).
The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 136 seconds
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Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml, Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
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Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml, Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
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Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/m, Green). The nuclear counterstain was DAPI (Blue). No staining on mouse liver.
Negative control: liver (PMID: 9001707) is observed.
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
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Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml, Green). The nuclear counterstain was DAPI (Blue). No staining on rat liver.
Negative control: liver (PMID: 9001707) is observed.
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml)
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Flow cytometric analysis of mouse primary neuron cells labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1µg / Right) compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
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Flow cytometric analysis of rat primary neuron cells labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1µg / Right) compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
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Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T (Human embryonic kidney epithelial cell) cells transfected with myc tagged human MGLUR3 construct labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1µg / Right), compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG or ab283572. Then fixed with 2% PFA for 10 minutes followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.
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Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Metabotropic glutamate receptor 3/MGLUR3 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab283572 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283572 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10µg
Lane 2: ab283572 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283572 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
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Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (ab283572)
Metabotropic glutamate receptor 3/MGLUR3 was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab283572 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283572 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10µg
Lane 2: ab283572 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab283572 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab283572 has not yet been referenced specifically in any publications.