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RecombinantRabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

Submit a review Submit a question References (3)
Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3
  • Suitable for: Flow Cyt, WB, ICC/IF, Indirect ELISA
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)]
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, Indirect ELISAmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human Metabotropic Glutamate Receptor 3/MGLUR3. The exact sequence is proprietary.
    Database link: Q14832
    (Peptide available as ab208352)

  • Positive control

    • WB: Mouse brain tissue lysate. Human cerebellum and hypothalamus tissue lysates; ICC/IF: Mouse primary neuron cells. Flow cyt: Mouse primary neuron and Neuro-2a cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
WB
1/1000 - 1/5000. Detects a band of approximately 110, 220 kDa (predicted molecular weight: 99 kDa).
ICC/IF
1/250.
Indirect ELISA
Use at an assay dependent concentration.
Notes
Flow Cyt
1/500.
WB
1/1000 - 1/5000. Detects a band of approximately 110, 220 kDa (predicted molecular weight: 99 kDa).
ICC/IF
1/250.
Indirect ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P or IP.

Target

Images

  • Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    ELISA analysis of Human MGLUR3 recombinant protein at 500ng/ml with ab166608. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 99 kDa



    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human hypothalamus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 220 kDa why is the actual band size different from the predicted?

  • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

References (3)

Publishing research using ab166608? Please let us know so that we can cite the reference in this datasheet.

ab166608 has been referenced in 3 publications.

  • Lee J  et al. Defining the Homo- and Heterodimerization Propensities of Metabotropic Glutamate Receptors. Cell Rep 31:107605 (2020). PubMed: 32375054
  • García-Bea A  et al. A group II metabotropic glutamate receptor 3 (mGlu3, GRM3) isoform implicated in schizophrenia interacts with canonical mGlu3 and reduces ligand binding. J Psychopharmacol 31:1519-1526 (2017). WB, ICC/IF . PubMed: 28655286
  • García-Bea A  et al. Metabotropic glutamate receptor 3 (mGlu3; mGluR3; GRM3) in schizophrenia: Antibody characterisation and a semi-quantitative western blot study. Schizophr Res N/A:N/A (2016). WB, ICC, IHC ; Mouse, Human . PubMed: 27130562

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