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    metabotropic-glutamate-receptor-3mglur3-antibody-epr90092-ab166608.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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RecombinantRabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

  • Datasheet
  • SDS
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Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
  • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3
  • Suitable for: Flow Cyt, WB, ICC/IF, Indirect ELISA
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody (ab53705)

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Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)]
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IF, Indirect ELISAmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Metabotropic Glutamate Receptor 3/MGLUR3. The exact sequence is proprietary.
    Database link: Q14832
    (Peptide available as ab208352)

  • Positive control

    • WB: Mouse brain tissue lysate. Human cerebellum and hypothalamus tissue lysates; ICC/IF: Mouse primary neuron cells. Flow cyt: Mouse primary neuron and Neuro-2a cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9009(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (ab249349)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
WB
1/1000 - 1/5000. Detects a band of approximately 110, 220 kDa (predicted molecular weight: 99 kDa).
ICC/IF
1/250.
Indirect ELISA
Use at an assay dependent concentration.
Notes
Flow Cyt
1/500.
WB
1/1000 - 1/5000. Detects a band of approximately 110, 220 kDa (predicted molecular weight: 99 kDa).
ICC/IF
1/250.
Indirect ELISA
Use at an assay dependent concentration.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14832 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2913 Human
    • Entrez Gene: 108069 Mouse
    • Omim: 601115 Human
    • SwissProt: Q14832 Human
    • SwissProt: Q9QYS2 Mouse
    • Unigene: 590575 Human
    • Unigene: 318966 Mouse
    • Alternative names

      • GLUR3 antibody
      • Glutamate metabotropic receptor 3 antibody
      • Glutamate receptor metabotropic 3 antibody
      • GPRC1C antibody
      • GRM3 antibody
      • GRM3_HUMAN antibody
      • Metabotropic glutamate receptor 3 antibody
      • MGlu3 antibody
      • mGluR3 antibody
      see all

    Images

    • Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      ELISA analysis of Human MGLUR3 recombinant protein at 500ng/ml with ab166608. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

    • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution + Mouse brain tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 99 kDa



      This blot was developed using a higher sensitivity ECL substrate.

    • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human hypothalamus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 99 kDa
      Observed band size: 220 kDa why is the actual band size different from the predicted?

    • Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab166608? Please let us know so that we can cite the reference in this datasheet.

    ab166608 has been referenced in 3 publications.

    • Lee J  et al. Defining the Homo- and Heterodimerization Propensities of Metabotropic Glutamate Receptors. Cell Rep 31:107605 (2020). PubMed: 32375054
    • García-Bea A  et al. A group II metabotropic glutamate receptor 3 (mGlu3, GRM3) isoform implicated in schizophrenia interacts with canonical mGlu3 and reduces ligand binding. J Psychopharmacol 31:1519-1526 (2017). WB, ICC/IF . PubMed: 28655286
    • García-Bea A  et al. Metabotropic glutamate receptor 3 (mGlu3; mGluR3; GRM3) in schizophrenia: Antibody characterisation and a semi-quantitative western blot study. Schizophr Res N/A:N/A (2016). WB, ICC, IHC ; Mouse, Human . PubMed: 27130562

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