Recombinant
RabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)

Submit a review Submit a question References (2)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)]
    See all Metabotropic Glutamate Receptor 3/MGLUR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR9009(2)] to Metabotropic Glutamate Receptor 3/MGLUR3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Metabotropic Glutamate Receptor 3/MGLUR3. The exact sequence is proprietary.
    Database link: Q14832
    (Peptide available as ab208352)

  • Positive control

    • Fetal brain, Human cerebellum and Human hypothalamus lysates

  • General notes

     

     

     This product was previously labelled as Metabotropic Glutamate Receptor 3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab166608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 110, 220 kDa (predicted molecular weight: 99 kDa).
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution + fetal brain lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 99 kDa
      Observed band size: 110& 220 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
      All lanes : Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608) at 1/1000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human hypothalamus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 99 kDa
      Observed band size: 220 kDa why is the actual band size different from the predicted?

    References (2)

    Publishing research using ab166608? Please let us know so that we can cite the reference in this datasheet.

    ab166608 has been referenced in 2 publications.

    • García-Bea A  et al. A group II metabotropic glutamate receptor 3 (mGlu3, GRM3) isoform implicated in schizophrenia interacts with canonical mGlu3 and reduces ligand binding. J Psychopharmacol 31:1519-1526 (2017). WB, ICC/IF . PubMed: 28655286
    • García-Bea A  et al. Metabotropic glutamate receptor 3 (mGlu3; mGluR3; GRM3) in schizophrenia: Antibody characterisation and a semi-quantitative western blot study. Schizophr Res N/A:N/A (2016). WB, ICC, IHC ; Mouse, Human . PubMed: 27130562

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