Overview

  • Product name
    Anti-Metabotropic Glutamate Receptor 4 antibody
    See all Metabotropic Glutamate Receptor 4 primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cat, Human
  • Immunogen

    Synthetic peptide derived from human Metabotropic Glutamate Receptor 4 (C-terminal)

  • Positive control
    • 293 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200.
WB 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
ELISA 1/5000.

Target

Images

  • Ab53088 staining Human cerebellum. Staining is localised to the cell membrane.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-Metabotropic Glutamate Receptor 4 antibody (ab53088) at 1/500 dilution

    Lane 1 : 293 cell extracts, treated
    with Forskolin (40nM, 30mins)
    Lane 2 : 293 cell extracts, treated
    with Forskolin (40nM, 30mins) with peptide

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • ab53088 staining the Metabotropic Glutamate Receptor 4 in Rat heart tissue sections by IHC-Fr (frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 1% Triton and blocked with 3% serum for 2 hours at 20°C. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab53088 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53088, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Novati A  et al. Environment-dependent striatal gene expression in the BACHD rat model for Huntington disease. Sci Rep 8:5803 (2018). Read more (PubMed: 29643462) »
  • Jenkins BG  et al. Functional modulation of G-protein coupled receptors during Parkinson disease-like neurodegeneration. Neuropharmacology 108:462-73 (2016). Read more (PubMed: 26581500) »
See all 5 Publications for this product

Customer reviews and Q&As

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1-10 of 18 Abreviews

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Cat Tissue sections (brainstem)
Antigen retrieval step
None
Permeabilization
Yes - 0.3 Triton
Specification
brainstem
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 10 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Cat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
None
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.2% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 8% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 07 2011

1-10 of 18 Abreviews

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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