Recombinant Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
![Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)](https://www.abcam.com/ps/products/184/ab184302/Images/ab184302-264684-anti-metabotropic-glutamate-receptor-4-antibody-epr19280-western-blot.jpg "Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280]
See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies -
Description
Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human, mouse and rat cerebellum lysates; mouse cerebellum lysate (2%SDS treated, not boiled). IP: Mouse and rat cerebellum lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19280 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/40.
|
|
| WB |
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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| Notes |
|---|
|
IP
1/40. |
|
WB
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. -
Tissue specificity
Strongly expressed in the cerebellum. Expressed at low levels in hippocampus, hypothalamus and thalamus. No expression detected in liver. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2914 Human
- Entrez Gene: 268934 Mouse
- Entrez Gene: 24417 Rat
- Omim: 604100 Human
- SwissProt: Q14833 Human
- SwissProt: Q68EF4 Mouse
- SwissProt: P31423 Rat
- Unigene: 654847 Human
see all -
Alternative names
- Glutamate receptor metabotropic 4 antibody
- GPRC1D antibody
- Grm4 antibody
see all
Images
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Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution
Lane 1 : Mouse cerebellum lysate
Lane 2 : Rat cerebellum lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Observed band size: ~300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
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![Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)](https://www.abcam.com/ps/products/184/ab184302/Images/ab184302-264683-anti-metabotropic-glutamate-receptor-4-antibody-epr19280-western-blot.jpg)
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution + Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 102 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
-
![Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)](https://www.abcam.com/ps/products/184/ab184302/Images/ab184302-264681-anti-metabotropic-glutamate-receptor-4-antibody-epr19280-western-blot.jpg)
Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 102 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.
-
![Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)](https://www.abcam.com/ps/products/184/ab184302/Images/ab184302-264680-anti-metabotropic-glutamate-receptor-4-antibody-epr19280-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse cerebellum lysate, 10μg (Input).
Lane 2: ab184302 IP in Mouse cerebellum lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)](https://www.abcam.com/ps/products/184/ab184302/Images/ab184302-264679-anti-metabotropic-glutamate-receptor-4-antibody-epr19280-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Rat cerebellum lysate, 10μg (Input).
Lane 2: ab184302 IP in Rat cerebellum lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)](https://www.abcam.com/ps/products/184/ab184302/Images/ab184302-6-benefits-of-recombinant-antibodies.png)
Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184302 has not yet been referenced specifically in any publications.