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    metabotropic-glutamate-receptor-4mglur4-antibody-epr19280-ab184302.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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RecombinantRabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
  • Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
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Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (ab166608)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
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Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody (ab53705)

View more associated products

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280]
    See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human, mouse and rat cerebellum lysates; mouse cerebellum lysate (2%SDS treated, not boiled). IP: Mouse and rat cerebellum lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19280
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (ab250743)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/40.
WB
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
Notes
IP
1/40.
WB
1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
  • Tissue specificity

    Strongly expressed in the cerebellum. Expressed at low levels in hippocampus, hypothalamus and thalamus. No expression detected in liver.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14833 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2914 Human
    • Entrez Gene: 268934 Mouse
    • Entrez Gene: 24417 Rat
    • Omim: 604100 Human
    • SwissProt: Q14833 Human
    • SwissProt: Q68EF4 Mouse
    • SwissProt: P31423 Rat
    • Unigene: 654847 Human
    • Unigene: 358940 Mouse
    • Unigene: 89046 Rat
    see all
  • Alternative names

    • Glutamate receptor metabotropic 4 antibody
    • GPRC1D antibody
    • Grm4 antibody
    • GRM4_HUMAN antibody
    • Metabotropic glutamate receptor 4 antibody
    • mGlu4 antibody
    • mGluR4 antibody
    see all

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

    Lane 1 : Mouse cerebellum lysate
    Lane 2 : Rat cerebellum lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa
    Observed band size: ~300 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution + Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 102 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.

  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

    Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse cerebellum lysate, 10μg (Input).

    Lane 2: ab184302 IP in Mouse cerebellum lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

    Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Rat cerebellum lysate, 10μg (Input).

    Lane 2: ab184302 IP in Rat cerebellum lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab184302? Please let us know so that we can cite the reference in this datasheet.

ab184302 has not yet been referenced specifically in any publications.

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