Recombinant
RabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280]
    See all Metabotropic Glutamate Receptor 4/MGLUR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19280] to Metabotropic Glutamate Receptor 4/MGLUR4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Mouse Metabotropic Glutamate Receptor 4/MGLUR4 aa 850 to the C-terminus. The exact sequence is proprietary.
    Database link: Q68EF4

  • Positive control

    • WB: Human, mouse and rat cerebellum lysates; mouse cerebellum lysate (2%SDS treated, not boiled). IP: Mouse and rat cerebellum lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab184302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
WB 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).

Target

Images

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

    Lane 1 : Mouse cerebellum lysate
    Lane 2 : Rat cerebellum lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa
    Observed band size: ~300 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution + Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 102 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.

  • Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    All lanes : Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 102 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID:8463825, PMID:22528491), though results may vary depending on percentage acrylamide gel used.

  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

    Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse cerebellum lysate, 10μg (Input).

    Lane 2: ab184302 IP in Mouse cerebellum lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)
    Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (ab184302)

    Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Rat cerebellum lysate, 10μg (Input).

    Lane 2: ab184302 IP in Rat cerebellum lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

Publishing research using ab184302? Please let us know so that we can cite the reference in this datasheet.

ab184302 has not yet been referenced specifically in any publications.

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