Overview

  • Product name
    Anti-Metabotropic Glutamate Receptor 5 antibody
    See all Metabotropic Glutamate Receptor 5 primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide within Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus. The exact sequence is proprietary.
    Database link: P41594

  • Positive control
    • Human brain for Immunohistochemistry.

Properties

Applications

Our Abpromise guarantee covers the use of ab27190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100. PubMed: 16979830Please note antigen retrieval is required.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 135 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutamate receptor metabotropic 5 antibody
    • GPRC1E antibody
    • Grm5 antibody
    • GRM5_HUMAN antibody
    • Metabotropic glutamate receptor 5 antibody
    • Metabotropic glutamate receptor 5 variant F antibody
    • Metabotropic glutamate receptor 5 variant G antibody
    • Metabotropic glutamate receptor 5 variant H antibody
    • mGlu5 antibody
    • mGluR5 antibody
    • PPP1R86 antibody
    • Protein phosphatase 1 regulatory subunit 86 antibody
    see all

Images

  • Ab27190, at a dilution of 1/50, staining mGluR5 in Paraffin embedded formalin fixed human brain tissue section.
  • ICC/IF image of ab27190 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27190, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

References

This product has been referenced in:
  • Guo Z  et al. Prenatal immune activation potentiates endocannabinoid-related plasticity of inhibitory synapses in the hippocampus of adolescent rat offspring. Eur Neuropsychopharmacol 28:1405-1417 (2018). Read more (PubMed: 30257799) »
  • Dai J  et al. Improved retinal function in RCS rats after suppressing the over-activation of mGluR5. Sci Rep 7:3546 (2017). Read more (PubMed: 28615682) »
See all 6 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Rat Recombinant protein (HEK cell transfected with rat mGluR5)
Loading amount
20 µg
Specification
HEK cell transfected with rat mGluR5
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Sriharsha Kantamneni

Verified customer

Submitted May 02 2007

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