Product nameAnti-Metabotropic Glutamate Receptor 5 antibody
See all Metabotropic Glutamate Receptor 5 primary antibodies
DescriptionRabbit polyclonal to Metabotropic Glutamate Receptor 5
Specificityab53090 detects endogenous levels of total Metabotropic Glutamate Receptor 5 protein.
Tested applicationsSuitable for: IHC-Fr, ICC/IF, IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human
N-terminal synthetic peptide derived from human Metabotropic Glutamate Receptor 5.
- WB: Mouse brain extracts IHC-P: Human brain tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab53090 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab53090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22962568|
|ICC/IF||1/100. PubMed: 19364772|
|IHC-P||1/50 - 1/100.|
|WB||1/300 - 1/1000. Predicted molecular weight: 132 kDa.|
FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- Glutamate receptor metabotropic 5 antibody
- GPRC1E antibody
- Grm5 antibody
All lanes : Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090) at 1/300 dilution
Lane 1 : Mouse brain extracts
Lane 2 : Mouse brain extracts with blocking peptide
Predicted band size: 132 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
ab53090 staining Metabotropic Glutamate Receptor 5 (red) in rat hippocampla neuron by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 22°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Cyan - pre-synaptic marker Bassoon.
Immunohistochemical analysis of paraffin embedded human brain tissue using ab53090 at 1/50 dilution, in the presence (right) and absence (left) of blocking peptide.
ab53090 staining Metabotropic Glutamate Receptor 5 in rat brain tissue sections by Immunohistochemistry (Free Floating).
Animals were perfused fixed with 4% paraformaldehyde with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using ab53090 at a 1/300 dilution. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit used at a 1/1000 dilution.
This product has been referenced in:
- Dai J et al. Improved retinal function in RCS rats after suppressing the over-activation of mGluR5. Sci Rep 7:3546 (2017). Read more (PubMed: 28615682) »
- Goniotaki D et al. Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity. PLoS Pathog 13:e1006733 (2017). Read more (PubMed: 29176838) »