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    metabotropic-glutamate-receptor-5-antibody-epr2425y-ab76316.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Glutamate Receptors
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RecombinantRabMAb

Recombinant Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

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Reviews (6)Q&A (2)References (31)

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Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
  • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5
  • Suitable for: Electron Microscopy, Flow Cyt (Intra), WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP

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Alexa Fluor® 647 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196929)
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Anti-mGluR1 antibody (ab82211)
Assay
Product image
Glutamate Assay Kit (ab83389)

View more associated products

Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
    See all Metabotropic Glutamate Receptor 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5
  • Host species

    Rabbit
  • Specificity

    The Human species recommendation is based on the WB results. This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.

  • Tested applications

    Suitable for: Electron Microscopy, Flow Cyt (Intra), WB, IHC-P, IHC-Frmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain lysate and Human hippocampus tissue. IHC-P: Rat caudate putamen and cerebrum tissue; mouse caudate putamen tissue and cerebrum IHC-Fr: Mouse caudate ptamen tissue. IHC (resin): Mouse neocortex tissue. EM: Mouse neocortex tissue. Flow Cyt (intra): SH-SY5Y cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2425Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Signal transduction
    • G protein signaling
    • GPCR
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196482)
    • Alexa Fluor® 647 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab196929)
    • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
    • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000 - 1/10000. Predicted molecular weight: 132 kDa.

Please do not boil the sample before loading to the gel.

IHC-P (2)
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species.

IHC-Fr (2)
Use a concentration of 1 µg/ml.

This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.

Notes
Electron Microscopy
Use at an assay dependent concentration.
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/5000 - 1/10000. Predicted molecular weight: 132 kDa.

Please do not boil the sample before loading to the gel.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species.

IHC-Fr
Use a concentration of 1 µg/ml.

This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.

Application notes
Is unsuitable for IP.

Target

  • Function

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41594 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2915 Human
    • Entrez Gene: 108071 Mouse
    • Entrez Gene: 24418 Rat
    • Omim: 604102 Human
    • SwissProt: P41594 Human
    • SwissProt: Q3UVX5 Mouse
    • SwissProt: P31424 Rat
    • Unigene: 147361 Human
    • Unigene: 616625 Human
    • Unigene: 235018 Mouse
    • Unigene: 29972 Rat
    see all
  • Alternative names

    • Glutamate receptor metabotropic 5 antibody
    • GPRC1E antibody
    • Grm5 antibody
    • GRM5_HUMAN antibody
    • Metabotropic glutamate receptor 5 antibody
    • Metabotropic glutamate receptor 5 variant F antibody
    • Metabotropic glutamate receptor 5 variant G antibody
    • Metabotropic glutamate receptor 5 variant H antibody
    • mGlu5 antibody
    • mGluR5 antibody
    • PPP1R86 antibody
    • Protein phosphatase 1 regulatory subunit 86 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    IHC image of Metabotropic Glutamate Receptor 5 staining in a section of frozen normal human hippocampus* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab76316, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    All lanes : Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution

    Lane 1 : Human spinal cord tissue lysate was not boiled before loading to the gel
    Lane 2 : Human hippocampus tissue lysate was not boiled before loading to the gel
    Lane 3 : Human liver tissue lysate was not boiled before loading to the gel
    Lane 4 : Human spinal cord tissue lysate was boiled before loading to the gel
    Lane 5 : Human hippocampus tissue lysate was boiled before loading to the gel
    Lane 6 : Human liver tissue lysate was boiled before loading to the gel

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 132 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?
    Additional bands at: 280 kDa (possible dimer)



    Buffer: 5% NFDM /TBST

    Please do not boil the sample before loading to the gel. 

    GRM5 is a brain enriched protein, can be observed in most of the brain areas but low in spinal cord (PMID: 12783878).

  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution + mouse brain lysate at 10 µg

    Secondary
    Goat anti-rabbit-HRP

    Developed using the ECL technique.

    Predicted band size: 132 kDa
    Observed band size: 140-150 kDa why is the actual band size different from the predicted?
    Additional bands at: >250 kDa (possible dimer)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
     
    Positive staining on mouse cerebrum.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on rat cerebrum.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)Image courtesy of Carl Hobbs, King College London, U.K.

    ab76316 staining Metabotropic Glutmate Receptor 5 in rat caudate putamen by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/2000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)Image courtesy of Carl Hobbs, King College London, U.K.

    ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde and samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/2000) for 16 hours at 21°C. An alexa fluor® 594 conjugated goat anti-rabbit IgG secondary was used at 1/500.

    See Abreview

  • Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)Images courtesy of Professor Richard Weinberg, UNC School of Medicine

    Postembedding immunogold labeling of mouse neocortex using Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316). The tissue was embedded in Lowicryl HM20 resin. 60 nm sections were then cut and mounted on nickel mesh grids before undergoing antigen retrieval for 15 minutes in 0.01 M citrate buffer, pH 6 at 60°C.

    The sections were then blocked in 1% BSA/TBSN pH 7.6 and incubated overnight at room temperature with Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1:250. Sections were washed twice in TBSN pH 7.6, treated with 1% normal donkey serum/TBSN pH 8.2 for 30 minutes, before being incubated with donkey anti-rabbit IgG-Au 10-20 nm at 1:20 for two hours at room temperature.

    Sections were then washed in TBSN pH 8.2, followed by water, before undergoing post-staining with 1% uranyl acetate and Sato’s lead. They were then air dried before being transferred to an oven for 30 minutes at 60°C.

    In these images you can see gold immunoparticles on the postsynaptic density of synapses.

    (TBSN = 0.02M TRIS buffered saline (0.3 N, pH 7.6 or 8.2) with 0.005% Tergitol NP-10)

  • Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)Image courtesy of Carl Hobbs, King College London, U.K.

    ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.

    See Abreview

  • Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
    Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (31)

Publishing research using ab76316? Please let us know so that we can cite the reference in this datasheet.

ab76316 has been referenced in 31 publications.

  • Huang M  et al. Deficiency of SHANK3 isoforms impairs thermal hyperalgesia and dysregulates the expression of postsynaptic proteins in the spinal cord. Neurosci Res 163:26-33 (2021). PubMed: 32147472
  • Chiprés-Tinajero GA  et al. Increased immunoreactivity of glutamate receptors, neuronal nuclear protein and glial fibrillary acidic protein in the hippocampus of epileptic rats with fast ripple activity. Exp Brain Res 239:2015-2024 (2021). PubMed: 33909110
  • Elhussiny MEA  et al. Modulation by chronic stress and ketamine of ionotropic AMPA/NMDA and metabotropic glutamate receptors in the rat hippocampus. Prog Neuropsychopharmacol Biol Psychiatry 104:110033 (2021). PubMed: 32640261
  • Peng K  et al. Mechanisms Underlying Enhancement of Spontaneous Glutamate Release by Group I mGluRs at a Central Auditory Synapse. J Neurosci 40:7027-7042 (2020). PubMed: 32801152
  • Wang Y  et al. mGluR5 mediates ketamine antidepressant response in susceptible rats exposed to prenatal stress. J Affect Disord 272:398-408 (2020). PubMed: 32553383
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 10 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS7)
Permeabilization
Yes - 0.2%Triton
Specification
COS7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 07 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Aug 11 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Aug 11 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Aug 11 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Rat Cell (rat hippocampal neuron)
Specification
rat hippocampal neuron
Permeabilization
Yes - 0.2%Triton
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 13 2014

Question


For a preadsorption with rabbit anti mGluR5 (ab76316 ) we are
interrested for an acccording peptide.

Read More

Abcam community

Verified customer

Asked on Jan 25 2013

Answer



I am happy to confirm that we have the suitable blocking peptide available in our catalog under the product number ab139974

https://www.abcam.com/Metabotropic-Glutamate-Receptor-5-peptide-ab139974.html (or use the following: https://www.abcam.com/Metabotropic-Glutamate-Receptor-5-peptide-ab139974.html).

Read More

Abcam Scientific Support

Answered on Jan 25 2013

Question

Hi,

Thank you for your email.

I believe the customer is interested in the peptide.

Could you please advise the details and also the pricing.

Hoping to hear back from you soon.

Kind regards,

Read More

Abcam community

Verified customer

Asked on Sep 28 2012

Answer

Thank you for your reply.

The blocking peptide for the Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is now available to purchase under the catalogue number ab139974. The details of this product can be viewed from the following link:

https://www.abcam.com/Metabotropic-Glutamate-Receptor-5-peptide-ab139974.html

If you have any further questions, please do not hesitate to ask.

Read More

Abcam Scientific Support

Answered on Sep 28 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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