Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 (HRP)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (HRP)
See all Metabotropic Glutamate Receptor 5 primary antibodies
DescriptionRabbit monoclonal [EPR2425Y] to Metabotropic Glutamate Receptor 5 (HRP)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Metabotropic Glutamate Receptor 5 aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
(Peptide available as
- IHC-P: Normal human cerebellum tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (Alexa Fluor® 488) (ab196481)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (Alexa Fluor® 647) (ab196929)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free (ab256589)
- Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)
Our Abpromise guarantee covers the use of ab196482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- Glutamate receptor metabotropic 5 antibody
- GPRC1E antibody
- Grm5 antibody
IHC image of Metabotropic Glutamate Receptor 5 staining in a section of formalin-fixed paraffin-embedded normal human cerebellum, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab196482 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab196482 has not yet been referenced specifically in any publications.