Key features and details
- Mouse monoclonal [N75/33] to Metabotropic Glutamate Receptor 5
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-Metabotropic Glutamate Receptor 5 antibody [N75/33]
See all Metabotropic Glutamate Receptor 5 primary antibodies
DescriptionMouse monoclonal [N75/33] to Metabotropic Glutamate Receptor 5
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein, corresponding to cytoplasmic, C terminal amino acids 824-1203 of Rat Metabotropic Glutamate Receptor 5 (NP_058708.1).
- Rat brain membrane tissue lysate.
The clone number has been updated from S75-33 to N75/33, both clone numbers name the same antibody clone.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab134271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 132 kDa.
1µg/ml of ab134271 is sufficient for detection of Metabotropic Glutamate Receptor 5 in 20µg of Rat brain membrane lysate.
FunctionG-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- Glutamate receptor metabotropic 5 antibody
- GPRC1E antibody
- Grm5 antibody
Anti-Metabotropic Glutamate Receptor 5 antibody [N75/33] (ab134271) at 1/1000 dilution + Rat brain membrane tissue lysate
Goat anti-mouse IgG HRP
Predicted band size: 132 kDa
Overlay histogram showing SH-SH5Y cells stained with ab134271 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134271, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134271 has not yet been referenced specifically in any publications.