Overview

Description

  • Nature

    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab139974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

  • Form

    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names

    • Glutamate receptor metabotropic 5
    • GPRC1E
    • Grm5
    • GRM5_HUMAN
    • Metabotropic glutamate receptor 5
    • Metabotropic glutamate receptor 5 variant F
    • Metabotropic glutamate receptor 5 variant G
    • Metabotropic glutamate receptor 5 variant H
    • mGlu5
    • mGluR5
    • PPP1R86
    • Protein phosphatase 1 regulatory subunit 86
    see all
  • Function

    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt

References

ab139974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



I am happy to confirm that we have the suitable blocking peptide available in our catalog under the product number ab139974

https://www.abcam.com/index.html?datasheet=139974 (or use the following: https://www.abcam.com/index.html?datasheet=139974).

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