Key features and details
- Mouse monoclonal [S75-3] to Metabotropic Glutamate Receptor 5b
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Product nameAnti-Metabotropic Glutamate Receptor 5b antibody [S75-3]
See all Metabotropic Glutamate Receptor 5b primary antibodies
DescriptionMouse monoclonal [S75-3] to Metabotropic Glutamate Receptor 5b
Specificityab236530 detects ~130kDa (both mGluR1 and mGluR5).
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Fusion protein corresponding to Rat Metabotropic Glutamate Receptor 5b aa 824-1203 (C terminal).
ILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGE TLRYKDRRLAQHKSEIECFTPKGSMGNGGRATMSSSNGKSVTWAQNEKST RGQHLWQRLSVHINKKENPNQTAVIKPFPKSTENRGPGAAAGGGSGPGVA GAGNAGCTATGGPEPPDAGPKALYDVAEAEESFPAAARPRSPSPISTLSH LAGSAGRTDDDAPSLHSETAARSSSSQGSLMEQISSVVTRFTANISELNS MMLSTAATPGPPGTPICSSYLIPKEIQLPTTMTTFAEIQPLPAIEVTGGA QGATGVSPAQETPTGAESAPGKPDLEELVALTPPSPFRDSVDSGSTTPNS PVSESALCIPSSPKYDTLIIRDYTQSSSSL
Database link: P31424
- WB: Rat brain membrane lysate. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab236530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 132 kDa.|
RelevanceGlutamate receptors constitute the principal excitatory neurotransmitter receptors in brain. Two classes of glutamate receptors exist: Ionotropic receptors, and metabotropic receptors (mGluRs). Metabotropic Glutamate Receptor 5b (GRM5) activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The metabotropic glutamate receptor type 5 (mGlu5) is expressed in two splice variants, mGlu5a and mGlu5b, which differ in that mGlu5b has a 33-amino acid insert in the intracellular C-terminal domain. This receptor subtype is highly regulated, with higher levels found in developing animals.
Cellular localizationCell Membrane
- Glutamate receptor, metabotropic 5 antibody
- GPRC1E antibody
- GRM5 antibody
Anti-Metabotropic Glutamate Receptor 5b antibody [S75-3] (ab236530) at 1/1000 dilution + Rat brain membrane lysate at 10 µg
Goat Anti-Mouse IgG HRP at 1/200 dilution
Predicted band size: 132 kDa
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Metabotropic Glutamate Receptor 5b (green) using ab236530 at 1/100 dilution, followed by Goat Anti-Mouse ATTO 488 at 1/200 dilution in ICC/IF. The nuclear counterstain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red). The merged image is shown in panel D. Localization shoen in Cell Membrane, Cytoplasm, and Nucleus.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236530 has not yet been referenced specifically in any publications.