Product nameAnti-Metabotropic Glutamate Receptor 7 antibody
See all Metabotropic Glutamate Receptor 7 primary antibodies
DescriptionRabbit polyclonal to Metabotropic Glutamate Receptor 7
Specificityab53705 detects endogenous levels of total Metabotropic Glutamate Receptor 7 protein
Tested applicationsSuitable for: IP, WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from human Metabotropic Glutamate Receptor 7.
- Human brain tissue and HuvEc cell extracts.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionReceptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
Tissue specificityExpressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family.
Cellular localizationCell membrane.
- Information by UniProt
- FLJ40498 antibody
- GLUR7 antibody
- Glutamate receptor metabotropic 7 antibody
Metabotropic Glutamate Receptor 7 was immunoprecipitated using 0.5mg Rat Brain tissue extract, 5µg of Rabbit polyclonal to Metabotropic Glutamate Receptor 7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53705.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 110kDa; Metabotropic Glutamate Receptor 7
ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
All lanes : Anti-Metabotropic Glutamate Receptor 7 antibody (ab53705) at 1/500 dilution
Lane 1 : HuvEc cell extract
Lane 2 : HuvEc cell extract with immunising peptide
Predicted band size: 102 kDa
Observed band size: 102 kDa