Overview

  • Product name

    Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody
    See all Metabotropic Glutamate Receptor 7/MGLUR7 primary antibodies
  • Description

    Rabbit polyclonal to Metabotropic Glutamate Receptor 7/MGLUR7
  • Host species

    Rabbit
  • Specificity

    ab53705 detects endogenous levels of total Metabotropic Glutamate Receptor 7/MGLUR7 protein

  • Tested applications

    Suitable for: IP, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 7/MGLUR7.

  • Positive control

    • Human brain tissue and HuvEc cell extracts.
  • General notes

     This product was previously labelled as Metabotropic Glutamate Receptor 7

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • Function

    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity.
  • Tissue specificity

    Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ40498 antibody
    • GLUR7 antibody
    • Glutamate receptor metabotropic 7 antibody
    • GPRC1G antibody
    • GRM7 antibody
    • GRM7_HUMAN antibody
    • Metabotropic glutamate receptor 7 antibody
    • MGLU7 antibody
    • mGluR7 antibody
    • OTTHUMP00000206961 antibody
    • OTTHUMP00000214674 antibody
    • OTTHUMP00000214675 antibody
    see all

Images

  • All lanes : Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody (ab53705) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • Metabotropic Glutamate Receptor 7/MGLUR7 was immunoprecipitated using 0.5mg Rat Brain tissue extract, 5µg of Rabbit polyclonal to Metabotropic Glutamate Receptor 7/MGLUR7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53705.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 110kDa; Metabotropic Glutamate Receptor 7/MGLUR7

  • ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7/MGLUR7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Commercial Peroxidase blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde

Dr. Sarah Franklin

Verified customer

Submitted Aug 14 2014

Answer

Thank you for contacting us.
The immunogen sequence is from C-terminal end.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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