Recombinant Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24530-11] to Metabotropic Glutamate Receptor 7/MGLUR7
- Suitable for: IHC-P, WB, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11]
See all Metabotropic Glutamate Receptor 7/MGLUR7 primary antibodies -
Description
Rabbit monoclonal [EPR24530-11] to Metabotropic Glutamate Receptor 7/MGLUR7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, IPmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Tissue lysates: Mouse and rat hippocampus, mouse spinal cord, SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate. IHC-P: Mouse and rat hippocampus, HEK-293T transfected with a Myc-His tagged GRM7 construct. IHC-Fr.: Rat hippocampus (fresh). IP: Tissue lysates: Mouse and rat hippocampus.
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General notes
That antibody is unsuitable for human IHC-P and mouse IHC-FR. Also, AB302530 does not react in ICC and intracellular flow cytometry respectively with human and mouse+rat species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24530-11 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 260,110 kDa (predicted molecular weight: 102 kDa).
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IHC-Fr |
1/50.
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IP |
1/30.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 260,110 kDa (predicted molecular weight: 102 kDa). |
IHC-Fr
1/50. |
IP
1/30. |
Target
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Function
Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. -
Tissue specificity
Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4. -
Sequence similarities
Belongs to the G-protein coupled receptor 3 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2917 Human
- Entrez Gene: 108073 Mouse
- Entrez Gene: 81672 Rat
- Omim: 604101 Human
- SwissProt: Q14831 Human
- SwissProt: Q68ED2 Mouse
- SwissProt: P35400 Rat
- Unigene: 606393 Human
see all -
Alternative names
- FLJ40498 antibody
- GLUR7 antibody
- Glutamate receptor metabotropic 7 antibody
see all
Images
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All lanes : Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530) at 1/1000 dilution
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse spinal cord tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse skeletal muscle tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : Rat hippocampus tissue lysate
Lane 7 : Rat heart tissue lysate
Lane 8 : Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 102 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Negative controls: heart, skeletal muscle, spleen (PMID: 12052533).
260-kDa GRM7 dimer is observed. The molecular weight is consistent with literature (PMID: 33767338, 19109906). -
Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530) at 1/1000 dilution + SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 102 kDa
Observed band size: 260 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate.
260-kDa GRM7 dimer is observed. The molecular weight is consistent with literature (PMID: 33767338, 19109906). -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 dilution (0.5 µg/mL) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus (PMID: 11007882, PMID: 9295396). The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 dilution (0.5 µg/mL) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hippocampus (PMID: 11007882, PMID: 9295396). The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) labeling Metabotropic Glutamate Receptor 7 / MGLUR7 with ab302530 at 1/500 dilution (1 µg/mL) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on (A) HEK-293T transfected with a Myc-His tagged GRM7 construct, no staining on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 dilution (0.5 µg/mL) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on mouse cardiac muscle. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
-
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 dilution (0.5 µg/mL) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on rat cardiac muscle. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus (fresh) tissue labeling Metabotropic Glutamate Receptor 7 / MGLUR7 with AB302530 at 1/50 dilution (10.02 µg/mL) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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I
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart (fresh) tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with AB302530 at 1/50 dilution (10.02 µg/mL)) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on rat heart is observed (PMID 12052533). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Metabotropic Glutamate Receptor 7/MGLUR7 was immunoprecipitated from 0.35 mg mouse hippocampus tissue lysate with AB302530 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB302530 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse hippocampus tissue lysate 10 µg (Inset)
Lane 2: AB302530 IP in Mouse hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302530 in mouse hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Observed MW (kDa) 260,110.
260-kDa GRM7 dimer and 110-kDa GRM7 monomer are observed (PMID: 33767338).
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Metabotropic Glutamate Receptor 7/MGLUR7 was immunoprecipitated from 0.35 mg rat hippocampus tissue lysate with AB302530 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using AB302530 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat hippocampus tissue lysate 10 µg (Inset)
Lane 2: AB302530 IP in rat hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of AB302530 in rat hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Observed MW (kDa): 260, 110.
260-kDa GRM7 dimer and 110-kDa GRM7 monomer are observed (PMID: 33767338).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302530 has not yet been referenced specifically in any publications.