Overview

  • Product name
    Anti-Metabotropic Glutamate Receptor 8 antibody
    See all Metabotropic Glutamate Receptor 8 primary antibodies
  • Description
    Rabbit polyclonal to Metabotropic Glutamate Receptor 8
  • Host species
    Rabbit
  • Specificity
    ab53094 detects endogenous levels of total Metabotropic Glutamate Receptor 8 protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from human Metabotropic Glutamate Receptor 8

  • Positive control
    • WB: Mouse brain extracts IHC-P: Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab53094 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Metabotropic Glutamate Receptor 8 antibody (ab53094) at 1/300 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Mouse brain extract with peptide

    Predicted band size: 102 kDa
    Observed band size: 102 kDa

  • Immunohistochemical analysis of paraffin-embedded human brain tissue in the presence (right) and absence (left) of blocking peptide, using ab53094 at 1/50 dilution.

References

This product has been referenced in:
  • Jantas D  et al. An endogenous and ectopic expression of metabotropic glutamate receptor 8 (mGluR8) inhibits proliferation and increases chemosensitivity of human neuroblastoma and glioma cells. Cancer Lett 432:1-16 (2018). Read more (PubMed: 29885518) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

The immunogen for this antibody is a fragment of amino acid 869 to 908 of human Metabotropic Glutamate Receptor 8.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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https://www.abcam.com/abreviews

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Answer

Thank you for your enquiry.

The immunogen information I provided you is more detailed than that provided on the website datasheet. Please note that, as the website states, the sequence I provided you is a "synthetic peptide derived from human Metabotropic Glutamate Receptor 8".

Often times we are able to provide more detailed immunogen information when requested. Asa business decision, the immunogensequences are not always provided directly on the datasheet.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer

The amino acid range of this immunogen is aa845-890 of human mGluR8 with accession number O00222 (http://www.uniprot.org/uniprot/O00222). This antibody binds to the C-terminal cytoplasmic domain of isoforms A and B. This antibody doesn't reacts with isoform C.

I hope this is helpful. Please contact us again if you have any further questions.

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https://www.abcam.com/abreviews

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Question
Answer

Thank you for contacting us.

The exact immunogen sequence corresponds to amino acid XXX to XXX.

We are runing a promotion on one of our products and givign away free. Please check the link and order

https://www.abcam.com/index.html?pageconfig=resource&rid=14968

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. Having checked our catalog for the appropriate products, I would like to send you the following recommendations. Where possible I looked for both monoclonal and polyclonal antibodies, so if not mentioned we unfortunately do not have monoclonals. I am happy to let you know that all tested species and applications are mentioned on the datasheet, and we can guarantee the antibody to work as indicated. In case the application IHC-P (or the species human) is not mentioned, the customer is eligible for our testing discount program:The discount would be to the value of 1 free primary antibody, if the customer would like to test the antibody in IHC-P/human (www.abcam.com/collaborationdiscount). 1. Anti-beta catenin: ab22656: Click here (or use the following: https://www.abcam.com/index.html?datasheet=22656). ab16051: Click here (or use the following: https://www.abcam.com/index.html?datasheet=16051). 2. GRM8 ab53094: Click here (or use the following: https://www.abcam.com/index.html?datasheet=53094). ab55067 (testing discount IHC-P): Click here (or use the following: https://www.abcam.com/index.html?datasheet=55067). 3. KCNA1 ab99010 (testing discount human): Click here (or use the following: https://www.abcam.com/index.html?datasheet=99010). ab32433: Click here (or use the following: https://www.abcam.com/index.html?datasheet=32433). 4. GLI1, SFRP1 and NPR3: ab92611 (no monoclonals available): Click here (or use the following: https://www.abcam.com/index.html?datasheet=92611). ab4193 (no monoclonals available): Click here (or use the following: https://www.abcam.com/index.html?datasheet=4193). ab123957: Click here (or use the following: https://www.abcam.com/index.html?datasheet=123957). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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