Overview

  • Product name

    Anti-Metallophilic Macrophages antibody [MOMA-1]
    See all Metallophilic Macrophages primary antibodies
  • Description

    Rat monoclonal [MOMA-1] to Metallophilic Macrophages
  • Host species

    Rat
  • Specificity

    No reactivity of ab183549 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells. Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by ab183549. No positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.
  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Metallophilic Macrophages. (Mouse lymph node tissue).

  • Positive control

    • Mouse spleen tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 98% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    MOMA-1
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr 1/200.

(2µg/ml).

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance

      Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.

    Images

    • Immunohistochemical analysis of frozen mouse spleen tissue labeling Metallophilic Macrophages with ab183549 at 1/200 dilution.

    References

    This product has been referenced in:

    • Nyström A  et al. Impaired lymphoid extracellular matrix impedes antibacterial immunity in epidermolysis bullosa. Proc Natl Acad Sci U S A 115:E705-E714 (2018). Read more (PubMed: 29305555) »
    • Jellusova J  et al. Gsk3 is a metabolic checkpoint regulator in B cells. Nat Immunol 18:303-312 (2017). Read more (PubMed: 28114292) »
    See all 2 Publications for this product

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