Key features and details
- Mouse monoclonal [2B5] to Metallothionein
- Suitable for: WB
- Reacts with: Pseudomonas aeruginosa
- Isotype: IgG1
Product nameAnti-Metallothionein antibody [2B5]
See all Metallothionein primary antibodies
DescriptionMouse monoclonal [2B5] to Metallothionein
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Pseudomonas aeruginosa
Recombinant full length protein corresponding to Pseudomonas aeruginosa Metallothionein.
Database link: Q9I1X5
- WB: Purified Pseudomonas aeruginosa Metallothionein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab240878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionMetallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Sequence similaritiesBelongs to the metallothionein superfamily. Type 1 family.
DomainClass I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
- Information by UniProt
- CES 1 antibody
- CES1 antibody
- Metallothionein 1A antibody
All lanes : Anti-Metallothionein antibody [2B5] (ab240878) at 1/1000 dilution
Lane 1 : GST tag
Lane 2 : Purified Pseudomonas aeruginosa Metallothionein GST tagged protein
Lysates/proteins at 1 µg per lane.
All lanes : HRP-linked goat anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 5 minutes
Blocked with 5% skimmed milk powder in TBST. Incubated with primary antibody for for 2 hours at RT and with secondary antibody for 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240878 has not yet been referenced specifically in any publications.