Overview

  • Product name

    Anti-Metallothionein antibody
    See all Metallothionein primary antibodies
  • Description

    Rabbit polyclonal to Metallothionein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Metallothionein aa 1 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: P02795

  • Positive control

    • This antibody gave a positive signal in Human Liver tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Normal human liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab134463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa).

Target

  • Function

    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
  • Sequence similarities

    Belongs to the metallothionein superfamily. Type 1 family.
  • Domain

    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
  • Information by UniProt
  • Database links

  • Alternative names

    • CES 1 antibody
    • CES1 antibody
    • Metallothionein 1A antibody
    • Metallothionein 1S antibody
    • Metallothionein 2 antibody
    • Metallothionein 2A antibody
    • Metallothionein IA antibody
    • Metallothionein II antibody
    • Metallothionein-1A antibody
    • Metallothionein-IA antibody
    • Metallothionein2 antibody
    • MGC32848 antibody
    • MT 1A antibody
    • MT 2 antibody
    • MT 2A antibody
    • MT IA antibody
    • MT II antibody
    • MT-1A antibody
    • MT-IA antibody
    • MT1 antibody
    • MT1A antibody
    • MT1A_HUMAN antibody
    • MT1S antibody
    • MT2 antibody
    • MT2A antibody
    • MTC antibody
    see all

Images

  • IHC image of Metallothionein staining in normal Human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab134463, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-Metallothionein antibody (ab134463) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 6 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 49 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    The predicted molecular weight of Metallothionein is 6 kDa (SwissProt), however we expect to observe a banding pattern around 15 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134463 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab134463 has not yet been referenced specifically in any publications.

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