The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Belongs to the metallothionein superfamily. Type 1 family.
Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
ab192385 staining Metallothionein in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with ab64226 for 10 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/150) for 30 minutes. An undiluted HRP-conjugated Goat polyclonal (1/250) was used as the secondary antibody
Western blot - Anti-Metallothionein antibody (ab192385)
Western blot analysis of extracts of HeLa cells useing ab192385.