Key features and details
- Rabbit polyclonal to Metallothionein
- Suitable for: ELISA, WB
- Isotype: IgG
Product nameAnti-Metallothionein antibody
See all Metallothionein primary antibodies
DescriptionRabbit polyclonal to Metallothionein
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with Atlantic cod (Gadus morhua) and with metallothionein from a wide variety of different species including whiting (Merlangius merlangus), yellow gurnard (Trigla lucerna) and flounder (Platichthys flesus) as well as some cypriniform, siluriform, salmoniform, perciform and rajiform species.
Metallothionein purified from liver of Atlantic cod (Gadus morhua).
- WB: Liver S9 fractions.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 1% BSA, 0.87% Sodium chloride
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab36882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/50 - 1/1000. Not recommended for coating due to the BSA content.|
|WB||1/50 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa).|
FunctionMetallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Sequence similaritiesBelongs to the metallothionein superfamily. Type 1 family.
DomainClass I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
- Information by UniProt
- CES 1 antibody
- CES1 antibody
- Metallothionein 1A antibody
Ab36882, at a concentration of 1 µg/ml, staining Metallothionein by ELISA. Coating buffer was used as a negative control.
All lanes : Anti-Metallothionein antibody (ab36882) at 1/500 dilution
Lane 2 : Purified metallothionein from Atlantic cod at 0.25 µg
Lane 3 : Liver S9 fractions from cadmium treated Atlantic cod at 2.5 µg
Lane 4 : Liver S9 fractions from cadmium treated Whiting at 2.5 µg
Lane 5 : Liver S9 fractions from cadmium treated Yellow gurnard at 2.5 µg
Lane 6 : Liver S9 fractions from cadmium treated Flounder at 2.5 µg
Predicted band size: 6 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Lane 1 represents the molecular weight standard.
ab36882 has been referenced in 1 publication.
- Yudkovski Y et al. Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium. Mar Environ Res 65:427-36 (2008). ELISA ; Fish . PubMed: 18342364