Overview

  • Product name
    Anti-Metallothionein antibody
    See all Metallothionein primary antibodies
  • Description
    Rabbit polyclonal to Metallothionein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Atlantic cod (Gadus morhua) and with metallothionein from a wide variety of different species including whiting (Merlangius merlangus), yellow gurnard (Trigla lucerna) and flounder (Platichthys flesus) as well as some cypriniform, siluriform, salmoniform, perciform and rajiform species.
  • Immunogen

    Metallothionein purified from liver of Atlantic cod (Gadus morhua).

  • Positive control
    • WB: Liver S9 fractions.

Properties

Applications

Our Abpromise guarantee covers the use of ab36882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50 - 1/1000. Not recommended for coating due to the BSA content.
WB 1/50 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 6 kDa).

Target

  • Function
    Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
  • Sequence similarities
    Belongs to the metallothionein superfamily. Type 1 family.
  • Domain
    Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
  • Information by UniProt
  • Alternative names
    • CES 1 antibody
    • CES1 antibody
    • Metallothionein 1A antibody
    • Metallothionein 1S antibody
    • Metallothionein 2 antibody
    • Metallothionein 2A antibody
    • Metallothionein IA antibody
    • Metallothionein II antibody
    • Metallothionein-1A antibody
    • Metallothionein-IA antibody
    • Metallothionein2 antibody
    • MGC32848 antibody
    • MT 1A antibody
    • MT 2 antibody
    • MT 2A antibody
    • MT IA antibody
    • MT II antibody
    • MT-1A antibody
    • MT-IA antibody
    • MT1 antibody
    • MT1A antibody
    • MT1A_HUMAN antibody
    • MT1S antibody
    • MT2 antibody
    • MT2A antibody
    • MTC antibody
    see all

Images

  • Ab36882, at a concentration of 1 µg/ml, staining Metallothionein by ELISA. Coating buffer was used as a negative control.
  • All lanes : Anti-Metallothionein antibody (ab36882) at 1/500 dilution

    Lane 2 : Purified metallothionein from Atlantic cod at 0.25 µg
    Lane 3 : Liver S9 fractions from cadmium treated Atlantic cod at 2.5 µg
    Lane 4 : Liver S9 fractions from cadmium treated Whiting at 2.5 µg
    Lane 5 : Liver S9 fractions from cadmium treated Yellow gurnard at 2.5 µg
    Lane 6 : Liver S9 fractions from cadmium treated Flounder at 2.5 µg

    Predicted band size: 6 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Lane 1 represents the molecular weight standard.

References

This product has been referenced in:
  • Yudkovski Y  et al. Quantitative immunochemical evaluation of fish metallothionein upon exposure to cadmium. Mar Environ Res 65:427-36 (2008). ELISA ; Fish . Read more (PubMed: 18342364) »
See 1 Publication for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your new contact.

My colleague is out of office today. I have attached the Western blot Questionnaire to this e-mail and would be grateful if you could complete it and send it back to us.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and will be very happy to investigate the antibody further.

- Please fill the attached questionnaire and provide images?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1148763.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab12228 in zebrafish in WB. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forzebrafish in WBand include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.


For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab12228.

To our knowledge, ab12228 has not been tested in zebrafish in WB. Therefore, I can offer a discount off a future purchase if you buy ab12228 now, test it inzebrafish and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab12228 in zebraifsh. I will then send a discount code. This code must be issued before purchasing ab12228 so please wait for my reply before ordering.

2. Purchase ab12228 either by phone, fax, or online (www.abcam.com).

3. Test it in zebrafish in WB.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab12228 turns out to be unsuitable for zebrafish, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us.

Indeed, we have three metallothionein antibodies.

Please follow the links:

https://www.abcam.com/index.html?datasheet=12228 (or use the following: https://www.abcam.com/index.html?datasheet=12228).

https://www.abcam.com/index.html?datasheet=36882 (or use the following: https://www.abcam.com/index.html?datasheet=36882).

https://www.abcam.com/index.html?datasheet=95042 (or use the following: https://www.abcam.com/index.html?datasheet=95042).


Please check the datasheet regarding tested application and species suitability.

Regarding the price, please select your country in the upper right corner, and then the price and the availability will be shown in the light blue panel right below the target name.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your enquiry. Metallothionein antibody (ab36882) is an antibody that can be applied and is guaranteed for use in ELISA. We do not sell specific kits for use with this antibody. However, the antibody can be applied relatively easily using a degree of optimisation. We guarantee this antibody for application in ELISA using direct and indirect ELISA (see protocol below for guidelines). We guarantee antibodies for use as a combination in sandwich ELISA only when stated on the antibody datasheets. The antibody itself is not dependent on the detection system that you employ and should be determined by the end user. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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