Anti-Metallothionein antibody [UC1MT] (ab12228)
Key features and details
- Mouse monoclonal [UC1MT] to Metallothionein
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Rabbit, Human
- Isotype: IgG1
Overview
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Product name
Anti-Metallothionein antibody [UC1MT]
See all Metallothionein primary antibodies -
Description
Mouse monoclonal [UC1MT] to Metallothionein -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, Flow Cytmore details -
Species reactivity
Reacts with: Rabbit, Human -
Immunogen
Full length protein corresponding to Rabbit Metallothionein. Cross-linked rabbit liver Metallothionein I and II.
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Positive control
- HeLa cell lysate treated with 100uM CdCl2 Rehydrated rabbit liver MTI/MTII
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2.68% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
UC1MT -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 6-20 kDa (predicted molecular weight: 6 kDa).
Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis. |
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 6-20 kDa (predicted molecular weight: 6 kDa). Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. -
Sequence similarities
Belongs to the metallothionein superfamily. Type 1 family. -
Domain
Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines. - Information by UniProt
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Database links
- Entrez Gene: 4489 Human
- Entrez Gene: 4502 Human
- Omim: 156350 Human
- Omim: 156360 Human
- SwissProt: P02795 Human
- SwissProt: P04731 Human
- SwissProt: P11957 Rabbit
- SwissProt: P18055 Rabbit
see all -
Alternative names
- CES 1 antibody
- CES1 antibody
- Metallothionein 1A antibody
see all
Images
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Anti-Metallothionein antibody [UC1MT] (ab12228) + Hela cell lysate
Secondary
HRP-conjugated antibody.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 6 kDa
Exposure time: 2 minutesThis image was generated using the ascites version of the product.
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ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution + Rabbit liver lysates
Predicted band size: 6 kDaThis image was generated using the ascites version of the product.
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ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab12228 has been referenced in 56 publications.
- Nie Y et al. Antitumor effects of cadmium against diethylnitrosamine-induced liver tumors in mice. Oncol Lett 23:33 (2022). PubMed: 34966449
- Tamano H et al. Metallothionein synthesis increased by Ninjin-yoei-to, a Kampo medicine protects neuronal death and memory loss after exposure to amyloid β1-42. J Pharm Health Care Sci 8:26 (2022). PubMed: 36316709
- Keenan J et al. Copper toxicity of inflection point in human intestinal cell line Caco-2 dissected: influence of temporal expression patterns. In Vitro Cell Dev Biol Anim 57:359-371 (2021). PubMed: 33559028
- Tamano H et al. Dehydroeffusol Pprevents Amyloid ß1-42-mediated Hippocampal Neurodegeneration via Reducing Intracellular Zn2+ Toxicity. Mol Neurobiol 58:3603-3613 (2021). PubMed: 33770339
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873