Anti-Metallothionein antibody [UC1MT] (ab12228)

Mouse monoclonal Metallothionein antibody [UC1MT]. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Rabbit, Horse, Dog, Human, Mummichug fish. Cited in 32 publication(s).

Overview

  • Product name
    Anti-Metallothionein antibody [UC1MT]
    See all Metallothionein primary antibodies
  • Description
    Mouse monoclonal [UC1MT] to Metallothionein
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Horse, Dog, Human, Mummichug fish
  • Immunogen

    Full length protein corresponding to Rabbit Metallothionein.

  • Positive control
    • HeLa cell lysate treated with 100uM CdCl2 Rehydrated rabbit liver MTI/MTII
  • General notes

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab12228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 6-20 kDa (predicted molecular weight: 6 kDa).

Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis.

Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.

Target

Images

  • Anti-Metallothionein antibody [UC1MT] (ab12228) + Hela cell lysate

    Secondary
    HRP-conjugated antibody.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 6 kDa


    Exposure time: 2 minutes


    This image was generated using the ascites version of the product.

  • ab12228 staining human uterus tissue at 10 ug/ml using IHC-P.

    This image was generated using the ascites version of the product.

  • ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution + Rabbit liver lysates

    Predicted band size: 6 kDa



    This image was generated using the ascites version of the product.

  • ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • All lanes : Anti-Metallothionein antibody [UC1MT] (ab12228)

    Lane 1 : Marker
    Lane 2 : MTI
    Lane 3 : MTII
    Lane 4 : Mummichug CdCl2

    Predicted band size: 6 kDa



    This image was generated using the ascites version of the product.

  • ab12228 staining catfish kidney tissue sections by IHC-Fr.  Sections were acetone fixed and blocked with a commercial blocking agent prior to incubation with the primary antibody, diluted 1/50, for 16 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/1000, was used as the secondary.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:
  • Kawahara B  et al. Carbon monoxide sensitizes cisplatin-resistant ovarian cancer cell lines toward cisplatin via attenuation of levels of glutathione and nuclear metallothionein. J Inorg Biochem 191:29-39 (2019). Read more (PubMed: 30458366) »
  • Rodríguez-Menéndez S  et al. The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells. Nutrients 10:N/A (2018). Read more (PubMed: 30513827) »
See all 34 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-5 of 5 Abreviews

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (15% acrylamide)
Sample
Human Cell lysate - whole cell (osteosarcoma)
Specification
osteosarcoma
Blocking step
blocking buffer Sigma as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 14 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 23°C
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Methanol
Fixative
Methanol

Abcam user community

Verified customer

Submitted Oct 27 2014

Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP (prostate cancer cell line))
Loading amount
10 µg
Specification
LNCaP (prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (7% tris-acetate gel)
Blocking step
Milk as blocking agent for 35 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jul 23 2008

Application
Immunohistochemistry (Frozen sections)
Sample
Catfish Tissue sections (kidney)
Specification
kidney
Fixative
Acetone
Permeabilization
No
Blocking step
Image-iT as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. David Matlack

Verified customer

Submitted Jun 09 2008

Application
Western blot
Sample
Rat Tissue lysate - whole (choroid plexus(CP) and kidney(K))
Loading amount
30 µg
Specification
choroid plexus(CP) and kidney(K)
Gel Running Conditions
Reduced Non-Denaturing (Native) (Gel 12,5%)
Blocking step
Casein as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 06 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up