Overview

  • Product name

    Anti-Methicillin Resistant Staphylococcus Aureus antibody [332/423]
    See all Methicillin Resistant Staphylococcus Aureus primary antibodies
  • Description

    Mouse monoclonal [332/423] to Methicillin Resistant Staphylococcus Aureus
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Staphylococcus aureus
  • Immunogen

    Native Staphylococcus aureus (methicillin resistant).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    332/423
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Methicillin-resistant Staphylococcus Aureus (MRSA) is a type of bacteria that is resistant to certain antibiotics. These antibiotics include methicillin and other more common antibiotics such as oxacillin, penicillin and amoxicillin. Staphylococcus infections, including MRSA, occur most frequently among persons in hospitals and healthcare facilities (such as nursing homes and dialysis centres) who have weakened immune systems.
  • Alternative names

    • MRSA antibody

References

This product has been referenced in:

  • Safavieh M  et al. Rapid Real-Time Antimicrobial Susceptibility Testing with Electrical Sensing on Plastic Microchips with Printed Electrodes. ACS Appl Mater Interfaces 9:12832-12840 (2017). Read more (PubMed: 28291334) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

Regarding the immunogens of the mentioned MRSA antibodies we have the following information:

ab62742: Unfortunately, more detailed information about the specific MRSA strain used to raise the antibody is unavailable. Please note that this antibody may cross-react with other strains of Staphylococcus aureus.

ab69237: Also, more detailed information on the immunogen of this antibody is unfortunately not available. It is not known whether the antibody can distinguish MRSA from other strains.

ab73263: The protein immunogen used in development of this antibody was purified from MRSA (strain EMRSA16), but the isoform(s) of penicillin-binding protein (PBP) was not determined. Bacteria usually express several isoforms of PBP, each form having a different molecular weight. In WB, the antibody detects a protein of ˜70 kDa, but we don't know if this is PBP2a or another form of PBP. The MRSA protein in not present in methicillin-susceptible Staphylococcus aureus, thus, the antibody recognises only methicillin-RESISTANT Staphylococcus aureus and does not react with methicillin-SENSITIVE Staphylococcus aureus. Furthermore, this antibody probably will not detect intact MRSA cells as to our knowledge the MRSA protein is not detectable on surfaces of intact cells.

We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. I hope this information is nevertheless helpful to our customer. Should you have any further questions, please do not hesitate to contact us.

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Question
Answer

Thank you for your reply. Yes, the ATCC MRSA strains are covered by the guarantee with these antibodies in the tested applications listed on each datasheet. Please let me know if you have any further questions and I'll be happy to help you.

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Answer

Thank you for your enquiry. The immunogen used to produce ab62742 is noted to be "Native Staphylococcus aureus (methicillin resistant)." The epitope has not been mapped to determine which protein on the surface of this cell line is the antigen for this antibody. I'm sorry I could not be more helpful in this instance. Please do not hesitate to contact us if you need anything further.

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