Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)


  • Product name
    Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]
    See all Methionine Aminopeptidase 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Methionine Aminopeptidase 2. The exact sequence is proprietary.

  • Positive control
    • HepG2, 293T, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Permeabilized 293T cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
      Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
    • Sequence similarities
      Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
    • Post-translational
      Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
    • Cellular localization
      Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • A930035J23Rik antibody
      • AI047573 antibody
      • AL024412 antibody
      • Amp2 antibody
      • AU014659, antibody
      • EIF 2 associated p67 homolog antibody
      • EIF-2-associated p67 antibody
      • Initiation factor 2 associated 67 kDa glycoprotein antibody
      • Initiation factor 2-associated 67 kDa glycoprotein antibody
      • MAP 2 antibody
      • MAP2 antibody
      • MAP2_HUMAN antibody
      • MetAP 2 antibody
      • Metap2 antibody
      • Methionine aminopeptidase 2 antibody
      • Methionyl aminopeptidase 2 antibody
      • MGC102452 antibody
      • MGC127390 antibody
      • MGC53792 antibody
      • MNPEP antibody
      • p67 antibody
      • p67eIF2 antibody
      • Peptidase M antibody
      • Peptidase M2 antibody
      see all


    • All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : C6 cell lysates
      Lane 5 : RAW 264.7 cell lysates
      Lane 6 : PC12 cell lysates
      Lane 7 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa
      Observed band size: 67 kDa (why is the actual band size different from the predicted?)

    • ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2 in permeabilized 293T cells by Flow cytometry (red) or a Rabbit IgG (negative control) (green).
    • ab124953 staining Methionine Aminopeptidase 2 in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/25 in PBS + 5% goat serum) for 1 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.


    This product has been referenced in:
    • Ng JY  et al. Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2. Biochem Biophys Res Commun 440:37-42 (2013). Read more (PubMed: 24041691) »

    See 1 Publication for this product

    Customer reviews and Q&As

    The immunogen for Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) was within the first 50 amino acids of the N terminus. So, the antibody will not recognize the truncated protein that is missing the 107 N-terminal amino acids.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Frozen sections)
    Mouse Tissue sections (Spleen from immune mouse)
    Spleen from immune mouse
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    Submitted Jan 08 2013


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